Human B7-H7/HHLA2 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human B7-H7/HHLA2 in direct ELISAs.
  
• Source
  Monoclonal Mouse IgG₁ Clone # 907812
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  Human embryonic kidney cell line HEK293-derived human B7-H7/HHLA2
  Met1-Asn344
  Accession # Q9UM44
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Endotoxin Level
  <0.10 EU per 1 μg of the antibody by the LAL method.
• Label
  Unconjugated

• Blockade of Receptor-ligand Interaction
  In a functional ELISA, 100-300 ng/mL of Mouse anti-Human B7-H7/HHLA2 Antibody (Catalog # MAB80841) will block 50% of the binding of 500 ng/mL of Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) to immobilized Recombinant Human TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR) coated at 125 ng/mL
  (100 µL/well). At 1.25 μg/mL, this antibody will block >90% of the binding.
  
• Immunocytochemistry
  8-25 µg/mL
  See below

Blockade of Receptor-ligand Interaction click image to view larger	TMIGD2/CD28H Binding to B7-H7/ HHLA2-transfected HEK293 Human Cell Line is Blocked by Human B7-H7/HHLA2 Antibody. In a functional flow cytometry test, biotinylated recombinant human TMIGD2/ CD28H (10 ng/mL, Catalog # 8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/ HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 μg/mL of Mouse Anti-Human B7-H7/ HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 μg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).
Blockade of Receptor-ligand Interaction click image to view larger	B7-H7 Binding to TMIGD2/ CD28H Blocked by Human B7-H7/HHLA2 Antibody. In a Surface Plasmon Resonance test, Recombinant Human TMIGD2/ CD28H Fc Chimera (Catalog # 8316-TR), coated at 10 ng/mL binds to Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7, 5 μg/mL) (blue line). Binding is completely blocked (red) by 50 μg/mL of Mouse Anti-Human B7-H7/ HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 isotype (Catalog # MAB002) and irrelevant mouse monoclonal antibody were used as controls and did not affect TMIGD2/ CD28H binding to B7-H7/HHLA2 (not shown).
Immunocytochemistry click image to view larger	B7-H7/HHLA2 in Human PBMCs. B7-H7/HHLA2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

• Long Name:
  B7 Homolog 7

• Entrez Gene IDs:
  11148 (Human); 102137704 (Cynomolgus Monkey)

• Alternate Names:
  B7H7; B7-H7; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2

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