Human BATF APC-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects Human BATF in ELISA and Western Blot.
  • Source
    Monoclonal Mouse IgG2B Clone # 687706
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human BATF
    Met1-Ser125
    Accession # Q16520
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of BATF in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human BATF APC‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8054A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BATF
BATF is a member of the AP-1 family of basic leucine zipper transcription factors. It associates with c-Jun proteins to form heterodimeric factors that inhibit transcription at AP-1 sites. The DNA binding of BATF is regulated by its phosphorylation at Ser43. BATF plays a key role in several aspects of immune system development. It suppresses the differentiation of NKT and iNKT cells, while it promotes the differentiation of Th17, Th2, follicular Th cells, CD8+ T cells, and CD8+ dendritic cells. It is required for class-switch recombination in B cells and T cells as well as for germinal center formation and B cell maturation. It is also required for the expression of T cell surface proteins that mediate homing of Th cells to the gut. BATF cooperates with IRF4 in binding to composite DNA elements that are responsive to both IRF4 and AP1. Human BATF1 shares 96% amino acid sequence identity with mouse and rat BATF1
  • Long Name:
    Activating Transcription Factor 1
  • Entrez Gene IDs:
    10538 (Human); 53314 (Mouse); 299206 (Rat)
  • Alternate Names:
    basic leucine zipper transcription factor, ATF-like; BATF; BATF1basic leucine zipper transcriptional factor ATF-like; B-ATFSFA2; B-cell-activating transcription factor; SFA2; SFA-2activating transcription factor B; SF-HT-activated gene 2 protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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