B cell maturation protein (BCMA) is a member of the TNF receptor superfamily (TNFRSF17). BCMA is expressed in immune organs and mature Bcell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. BCMA binding to APRIL or BAFF stimulates IgM production in peripheral blood B cells and increases the survival of cultured B cells. Mature human and mouse BCMA share 86% amino acid sequence identity.
Human BCMA/TNFRSF17 Quantikine ELISA Kit
R&D Systems | Catalog # DBCMA0
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (50 µL), Urine (50 µL)
Sensitivity
10.8 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
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Product Summary for Human BCMA/TNFRSF17 Quantikine ELISA Kit
The Quantikine Human B Cell Maturation Antigen (BCMA) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BCMA in cell culture supernates, serum, plasma, saliva, and urine. It contains recombinant human BCMA and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BCMA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BCMA.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human BCMA.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 210 | 700 | 1308 | 211 | 659 | 1314 |
| Standard Deviation | 9.49 | 22.2 | 43.6 | 10.2 | 48.1 | 73.4 |
| CV% | 4.5 | 3.2 | 3.3 | 4.8 | 7.3 | 5.6 |
Recovery for Human BCMA/TNFRSF17 Quantikine ELISA Kit
The recovery of human BCMA spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 80-106 |
| Saliva (n=4) | 96 | 83-115 |
| Urine (n=4) | 102 | 94-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human BCMA were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human BCMA/TNFRSF17 Quantikine ELISA Kit
Human BCMA/TNFRSF17 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BCMA/TNFRSF17
Long Name
B Cell Maturation Factor
Alternate Names
CD269, TNFRSF13A, TNFRSF17
Gene Symbol
TNFRSF17
Additional BCMA/TNFRSF17 Products
Product Documents for Human BCMA/TNFRSF17 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human BCMA/TNFRSF17 Quantikine ELISA Kit
For research use only
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Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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