Human beta 2-Microglobulin Quantikine IVD ELISA Kit

Newer Version Available: KGE019
For In Vitro Diagnostic Use, Not Available in the EU

Discontinued Product

DBM200 has been discontinued and is replaced by KGE019.

beta 2-Microglobulin ELISA Kit ELISA
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Product Details
Procedure
Citations (13)
FAQs
Reviews (2)

Human beta 2-Microglobulin Quantikine IVD ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
1.25 hours
Sample Type & Volume Required Per Well
Serum (20 uL), Urine (20 uL)
Sensitivity
0.2 ug/mL
Assay Range
0.4 - 12 ug/mL (Serum, Urine)
Specificity
Measures human beta 2-Microglobulin levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with other substances.

Product Summary

The Quantikine IVD Human beta 2-Microglobulin Immunoassay is designed for the quantitative determination of beta 2-Microglobulin concentration in human serum and urine as an aid in the diagnosis of active rheumatoid arthritis and kidney diseases. For in vitro diagnostic use. Not available in the EU.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Samples of known concentration were tested in separate assays to assess inter-assay precision

Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3 4 5 6
n 10 10 10 40 40 40 40 40 40
Mean 0.9 1.4 8.4 0.9 1.3 8.9 1.1 3.2 8
Standard Deviation 0 0 0 0 0 0 0 0 0
CV% 8.1 6.3 5.3 9.2 6.8 4.8 8.9 6.9 6

Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 10 10 10 40 40 40
Mean 1.2 3.3 8.6 1.1 3.4 8.7
Standard Deviation 0 0 0 0 0 0
CV% 7.4 5.3 4.4 9.2 5.6 4.9

Recovery

The recovery of human beta 2-Microglobulin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=) 98 86.7-113.3
Urine (n=) 105 86.7-113.3

Linearity

Five samples were assayed at serial two-fold dilutions. The data below are typical of the assay.
Human beta 2-Microglobulin In Vitro Diagnostic ELISA Linearity

Data Examples

Human beta 2-Microglobulin In Vitro Diagnostic Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta 2-Microglobulin

beta 2-Microglobulin (B2M) forms the light chain of Class I MHC molecules. It has been measured in a variety of body fluids, including serum/plasma, saliva, CSF, and urine. B2M freely passes through the glomerular membrane, but it is nearly completely reabsorbed and degraded in proximal tubule cells. Clinically, serum B2M is elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions associated with decreased glomerular filtration.

Entrez Gene IDs:
567 (Human); 12010 (Mouse); 24223 (Rat)
Alternate Names:
B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use.
  1.   Prepare all reagents as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 20 µL Standard, Control, or Sample
  4.   Add 20 µL of Standard, Control, or sample to the appropriate wells.

  5. 100 µL Conjugate Solution
  6.   Add 100 µL of Conjugate Solution to each well.

  7. 100 µL Antibody Solution
  8.   Add 100 µL of Antibody Solution to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
  9.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.

  10. 100 µL Substrate Solution
  11.   Add 100 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 15 minutes.

  12. 100 µL Stop Solution
  13.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 620 nm.

Citations for Human beta 2-Microglobulin Quantikine IVD ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Augmented efficacy with the combination of blockade of the Notch-1 pathway, bortezomib and romidepsin in a murine MT-1 adult T-cell leukemia model.
    Authors: Yu P, Petrus M, Ju W, Zhang M, Conlon K, Nakagawa M, Maeda M, Bamford R, Waldmann T
    Leukemia, 2015;29(3):556-66.
    Species: Human
    Sample Types: Serum
  2. Anti-beta(2)M monoclonal antibodies kill myeloma cells via cell- and complement-mediated cytotoxicity.
    Authors: Zhang M, Qian J, Lan Y, Lu Y, Li H, Hong B, Zheng Y, He J, Yang J, Yi Q
    Int J Cancer, 2014;135(5):1132-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Indomethacin reduces glomerular and tubular damage markers but not renal inflammation in chronic kidney disease patients: a post-hoc analysis.
    Authors: de Borst MH, Nauta FL, Vogt L, Laverman GD, Gansevoort RT, Navis G
    PLoS ONE, 2012;7(5):e37957.
    Species: Human
    Sample Types: Urine
  4. beta2-microglobulin induces epithelial to mesenchymal transition and confers cancer lethality and bone metastasis in human cancer cells.
    Authors: Josson S, Nomura T, Lin JT, Huang WC, Wu D, Zhau HE, Zayzafoon M, Weizmann MN, Gururajan M, Chung LW
    Cancer Res., 2011;71(7):2600-10.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Effective treatment of a murine model of adult T-cell leukemia using depsipeptide and its combination with unmodified daclizumab directed toward CD25.
    Authors: Chen J, Zhang M, Ju W, Waldmann TA
    Blood, 2009;113(6):1287-93.
    Species: Mouse
    Sample Types: Serum
  6. Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Johnson D, Hathout Y, Ray PE
    Clin J Am Soc Nephrol, 2009;4(4):763-71.
    Species: Human
    Sample Types: Urine
  7. Gene modulatory effects, pharmacokinetics, and clinical tolerance of interferon-alpha1b: a second member of the interferon-alpha family.
    Authors: Masci P, Olencki T, Wood L, Rybicki L, Jacobs B, Williams B, Faber P, Bukowski R, Tong K, Borden EC
    Clin. Pharmacol. Ther., 2007;81(3):354-61.
    Species: Human
    Sample Types: Serum
  8. Beta-2-microglobulin is an androgen-regulated secreted protein elevated in serum of patients with advanced prostate cancer.
    Authors: Gross M, Top I, Laux I, Katz J, Curran J, Tindell C, Agus D
    Clin. Cancer Res., 2007;13(7):1979-86.
    Species: Human
    Sample Types: Serum
  9. Effective treatment of a murine model of adult T-cell leukemia using 211At-7G7/B6 and its combination with unmodified anti-Tac (daclizumab) directed toward CD25.
    Authors: Zhang Z, Zhang M, Garmestani K, Talanov VS, Plascjak PS, Beck B, Goldman C, Brechbiel MW, Waldmann TA
    Blood, 2006;108(3):1007-12.
    Species: Human
    Sample Types: Serum
  10. beta2-microglobulin is a signaling and growth-promoting factor for human prostate cancer bone metastasis.
    Authors: Huang WC, Wu D, Xie Z, Zhau HE, Nomura T, Zayzafoon M, Pohl J, Hsieh CL, Weitzmann MN, Farach-Carson MC, Chung LW
    Cancer Res., 2006;66(18):9108-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Effective therapy for a murine model of human anaplastic large-cell lymphoma with the anti-CD30 monoclonal antibody, HeFi-1, does not require activating Fc receptors.
    Authors: Zhang M, Yao Z, Zhang Z, Garmestani K, Goldman CK, Ravetch JV, Janik J, Brechbiel MW, Waldmann TA
    Blood, 2006;108(2):705-10.
    Species: Human
    Sample Types: Serum
  12. Changes in HIV RNA viral load, CD4+ T-cell counts, and levels of immune activation markers associated with anti-tuberculosis therapy and cotrimoxazole prophylaxis among HIV-infected tuberculosis patients in Abidjan, Cote d'Ivoire.
    Authors: Kalou M, Sassan-Morokro M, Abouya L, Bile C, Maurice C, Maran M, Tossou O, Roels T, Greenberg AE, Wiktor SZ, Nkengasong JN
    J. Med. Virol., 2005;75(2):202-8.
    Species: Human
    Sample Types: Serum
  13. Activating Fc receptors are required for antitumor efficacy of the antibodies directed toward CD25 in a murine model of adult t-cell leukemia.
    Authors: Zhang M, Zhang Z, Garmestani K, Goldman CK, Ravetch JV, Brechbiel MW, Carrasquillo JA, Waldmann TA
    Cancer Res., 2004;64(16):5825-9.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human beta 2-Microglobulin Quantikine IVD ELISA Kit

Average Rating: 3.5 (Based on 2 Reviews)

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Human beta 2-Microglobulin Quantikine IVD ELISA Kit
By Anonymous on 11/07/2016
Sample Tested: Urine

Human beta 2-Microglobulin Quantikine IVD ELISA Kit
By Anonymous on 08/15/2016
Sample Tested: Human Dialysate

I use the kit for twom times, each used half plate. The first one is excelent, standards and QC are all in the right range and low deviation. However for the second assay, the low QC is out of range and the highest standard is bout 35% bias.