beta 2-Microglobulin (B2M) forms the light chain of Class I MHC molecules. It has been measured in a variety of body fluids, including serum/plasma, saliva, CSF, and urine. B2M freely passes through the glomerular membrane, but it is nearly completely reabsorbed and degraded in proximal tubule cells. Clinically, serum B2M is elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions associated with decreased glomerular filtration.
Human beta 2-Microglobulin Quantikine IVD ELISA Kit
R&D Systems | Catalog # DBM200
For In Vitro Diagnostic Use, Not Available in the EU.
Discontinued Product
DBM200 has been discontinued.
An alternative/replacement product is available:
KGE019. View all beta 2-Microglobulin products.
Key Product Details
Assay Length
1.25 hours
Sample Type & Volume Required Per Well
Serum (20 µL), Urine (20 µL)
Sensitivity
0.2 µg/mL
Assay Range
0.4-12 µg/mL (Serum, Urine)
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Product Summary for Human beta 2-Microglobulin Quantikine IVD ELISA Kit
The Quantikine IVD Human beta 2-Microglobulin Immunoassay is designed for the quantitative determination of beta 2-Microglobulin concentration in human serum and urine as an aid in the diagnosis of active rheumatoid arthritis and kidney diseases. For in vitro diagnostic use. Not available in the EU.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Measures human beta 2-Microglobulin levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with other substances.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum
| Intra-Assay Precision | Inter-Assay Precision | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 |
| n | 10 | 10 | 10 | 40 | 40 | 40 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.9 | 1.4 | 8.4 | 0.9 | 1.3 | 8.9 | 1.1 | 3.2 | 8.0 |
| Standard Deviation | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| CV% | 8.1 | 6.3 | 5.3 | 9.2 | 6.8 | 4.8 | 8.9 | 6.9 | 6.0 |
Urine
| Intra-Assay Precision | Inter-Assay Precision | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 | |||
| n | 10 | 10 | 10 | 40 | 40 | 40 | |||
| Mean (pg/mL) | 1.2 | 3.3 | 8.6 | 1.1 | 3.4 | 8.7 | |||
| Standard Deviation | 0 | 0 | 0 | 0 | 0 | 0 | |||
| CV% | 7.4 | 5.3 | 4.4 | 9.2 | 5.6 | 4.9 | |||
Recovery for Human beta 2-Microglobulin Quantikine IVD ELISA Kit
The recovery of human beta 2-Microglobulin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Serum (n=0) | 98 | 86.7-113.3 |
| Urine (n=0) | 105 | 86.7-113.3 |
Linearity
Five samples were assayed at serial two-fold dilutions. The data below are typical of the assay.
Scientific Data Images for Human beta 2-Microglobulin Quantikine IVD ELISA Kit
Human beta 2-Microglobulin In Vitro Diagnostic Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: beta 2-Microglobulin
Alternate Names
B2M, beta 2Microglobulin
Gene Symbol
B2M
Additional beta 2-Microglobulin Products
Product Documents for Human beta 2-Microglobulin Quantikine IVD ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Related Research Areas
Citations for Human beta 2-Microglobulin Quantikine IVD ELISA Kit
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Customer Reviews for Human beta 2-Microglobulin Quantikine IVD ELISA Kit (2)
3.5 out of 5
2 Customer Ratings
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Sample Tested: UrineVerified Customer | Posted 11/07/2016
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Sample Tested: Human DialysateVerified Customer | Posted 08/15/2016I use the kit for twom times, each used half plate. The first one is excelent, standards and QC are all in the right range and low deviation. However for the second assay, the low QC is out of range and the highest standard is bout 35% bias.
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Protocols
View specific protocols for Human beta 2-Microglobulin Quantikine IVD ELISA Kit (DBM200):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use.
- Prepare all reagents as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 20 µL of Standard, Control, or sample to the appropriate wells.
- Add 100 µL of Conjugate Solution to each well.
- Add 100 µL of Antibody Solution to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
- Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.
- Add 100 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 15 minutes.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 620 nm.
20 µL Standard, Control, or Sample
100 µL Conjugate Solution
100 µL Antibody Solution
100 µL Substrate Solution
100 µL Stop Solution
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