Human beta 2-Microglobulin Parameter Assay Kit

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KGE019
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Human beta 2-Microglobulin Parameter Assay Kit Summary

Assay Length
1.5 hours
Sample Type & Volume Required Per Well
Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Urine (10 uL)
Sensitivity
0.132 ug/mL
Assay Range
0.2 - 10 ug/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Measures Human beta 2M levels in various samples.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Parameter beta 2-Microglobulin Immunoassay is a 1.5 hour forward sequential competitive enzyme immunoassay designed to measure beta 2M in serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three controls of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three controls of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.14 3.19 6.41 0.953 3.1 6.17
Standard Deviation 0.086 0.189 0.383 0.175 0.293 0.496
CV% 7.5 5.9 6 18.4 9.5 8

Recovery

The recovery of human beta 2M spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 79-114
Heparin Plasma (n=4) 106 91-124
Serum (n=4) 101 85-122
Urine (n=4) 90 76-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human beta 2M were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta 2-Microglobulin

beta 2-Microglobulin (B2M) forms the light chain of Class I MHC molecules. It has been measured in a variety of body fluids, including serum/plasma, saliva, CSF, and urine. B2M freely passes through the glomerular membrane, but it is nearly completely reabsorbed and degraded in proximal tubule cells. Clinically, serum B2M is elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions associated with decreased glomerular filtration.

Entrez Gene IDs:
567 (Human); 12010 (Mouse); 24223 (Rat)
Alternate Names:
B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

FAQs

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Human beta 2-Microglobulin Parameter Assay Kit
By Anonymous on 08/23/2016
Sample Tested: Human Dialysate