Human beta 2-Microglobulin Parameter Assay Kit

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  • Assay Length
    1.5 hours
  • Sample Type & Volume Required Per Well
    Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Urine (10 uL)
  • Sensitivity
    0.132 ug/mL
  • Assay Range
    0.2 - 10 ug/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Measures Human beta 2M levels in various samples.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Parameter beta 2-Microglobulin Immunoassay is a 1.5 hour forward sequential competitive enzyme immunoassay designed to measure beta 2M in serum, plasma, and urine.

Intra-Assay Precision (Precision within an assay) Three controls of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three controls of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0860.1890.3830.1750.2930.496


The recovery of human beta 2M spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 79-114
Heparin Plasma (n=4) 106 91-124
Serum (n=4) 101 85-122
Urine (n=4) 90 76-105
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human beta 2M were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human beta 2-Microglobulin Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: beta 2-Microglobulin
beta 2-Microglobulin (B2M) forms the light chain of Class I MHC molecules. It has been measured in a variety of body fluids, including serum/plasma, saliva, CSF, and urine. B2M freely passes through the glomerular membrane, but it is nearly completely reabsorbed and degraded in proximal tubule cells. Clinically, serum B2M is elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions associated with decreased glomerular filtration.
    • Entrez Gene IDs
      567 (Human); 12010 (Mouse); 24223 (Rat);
    • Alternate Names
      B2M; beta 2Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin;
    Related Research Areas
    Average Rating: 4 (Based on 1 review)

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      Anonymous 08/23/2016


    Sample TestedHuman Dialysate

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