Human Bora Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Bora

Bora (Aurora borealis; also C13orf34) is a 61 kDa member of the Bora family of proteins.  It is ubiquitously expressed, and plays a key role in cell cycle progression.  Plk1 (polo-like kinase-1) is a phosphorylase that is important to the cell during the G2/M transition and mitosis.  Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on Thr210.  Bora, Aurora-A and Plk1 all appear to form a complex during G2.  Bora predisposes Plk1 to the actions of Aurora-A.  Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora.  This initiates BORA dissociation from Aurora-A with subsequent ubiquitination and degradation.  Human Bora is 559 amino acids (aa) in length. It contains a Ser-rich region (aa 188-278) and at least eight utilized Ser phosphorylation sites.  Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85 kDa.  There is one potential alternative start site that lies 60 aa upstream of the standard start site, and a second splice variant the shows a 17 aa substitution for aa 1-87.  Over aa 2-180, human Bora shares 84% aa identity with mouse Bora.

Product Datasheets

Related Research Areas

• Checkpoint