Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 971526
View all BPI Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK-293-derived transfected with human BPI
Val32-Lys487
Accession # P17213

Specificity

Detects human BPI in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human BPI Alexa Fluor® 488‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: HL-60 Human acute promyelocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BPI

Bactericidal/Permeability Increasing protein (BPI) is a 55 kDa antibacterial glycoprotein that plays a role in innate immunity (1, 2). It belongs to the lipid transfer protein family that also includes LPS binding protein (LBP), cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Circulating levels of BPI are positively correlated with the levels of cholesterol, LDL cholesterol, and HDL cholesterol (3). Mature human BPI shares approximately 55% amino acid (aa) sequence identity with mouse and rat BPI. It can be seceted as a monomer or as a disulfide‑linked homodimer (4). It consists of a highly basic N‑terminal and a hydrophobic C‑terminal domain (5). Its N‑terminal domain confers the ability of BPI to bind bacterial lipopolysaccharide (LPS) found in the cell walls of Gram negative bacteria and to induce the lysis and phagocytosis of these bacteria (6‑9). It also blocks the endothelial cell response to endotoxin (10). BPI is stored in neutrophil and eosinophil granules for induced secretion during inflammation (11, 12). It is additionally expressed in mucosal epithelia and testis (10, 13). BPI can be retained on the surface of both neutrophils and epithelial cells, presumably by its hydrophobic C‑terminal domain (8, 10). BPI also functions as an anti-angiogenic molecule by inhibiting vascular endothelial cell proliferation and tubule formation (14). Like the antibacterial actions, this function is mediated by the N‑terminal region (15).

References

  1. Schultz, H. and J.P. Weiss (2007) Clin. Chim. Acta 384:12.
  2. Holweg, A. et al. (2011) Biochem. Soc. Trans. 39:1045.
  3. Esteve, E. et al. (2010) Thromb. Haemost. 103:780.
  4. Horwitz, A.H. et al. (1996) Protein Exp. Purif. 8:28.
  5. Gray, P.W. et al. (1989) J. Biol. Chem. 264:9505.
  6. Ooi, C.E. et al. (1987) J. Biol. Chem. 262:14891.
  7. Tobias, P.S. et al. (1997) J. Biol. Chem. 272:18682.
  8. Weersink, A.J. et al. (1993) J. Immunol. 150:253.
  9. Nishimura, H. et al. (2001) Immunology 103:519.
  10. Canny, G. et al. (2002) Proc. Natl. Acad. Sci. 99:3902.
  11. Weiss, J. and I. Olsson (1987) Blood 69:652.
  12. Calafat, J. et al. (1998) Blood 91:4770.
  13. Lennartsson, A. et al. (2005) J. Leukoc. Biol. 77:369.
  14. van der Schaft, D.W.J. et al. (2000) Blood 96:176.
  15. Rauniyar, R.K. et al. (2002) Invest. Ophthalmol. Vis. Sci. 43:503.

Long Name

Bactericidal/Permeability-increasing Protein

Alternate Names

BPIFD1, CAP 57, rBPI

Entrez Gene IDs

671 (Human); 329547 (Mouse); 296321 (Rat)

Gene Symbol

BPI

UniProt

P17213 (Human)

Additional BPI Products

Product Documents for Human BPI Alexa Fluor® 488‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human BPI Alexa Fluor® 488‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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