Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 971526
View all BPI Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK-293-derived transfected with human BPI
Val32-Lys487
Accession # P17213

Specificity

Detects human BPI in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human BPI Antibody

Detection of Human BPI antibody by Western Blot.

Detection of Human BPI by Western Blot.

Western blot shows lysates of Human bone marrow and human spleen tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human BPI Monoclonal Antibody (Catalog # MAB74681) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for BPI at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BPI antibody in Human Tonsil by Immunohistochemistry (IHC-P).

BPI in Human Tonsil.

BPI was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human BPI Monoclonal Antibody (Catalog # MAB74681) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of BPI antibody in HL-60 Human Cell Line antibody by Flow Cytometry.

Detection of BPI in HL-60 Human Cell Line by Flow Cytometry.

HL-60 human acute promyelocytic leukemia cell line was stained with Mouse Anti-Human BPI Monoclonal Antibody (Catalog # MAB74681, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human BPI Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HL-60 Human acute promyelocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Western Blot

2 µg/mL
Sample: Human bone marrow and human spleen tissue

Reviewed Applications

Read 1 review rated 5 using MAB74681 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BPI

Bactericidal/Permeability Increasing protein (BPI) is a 55 kDa antibacterial glycoprotein that plays a role in innate immunity (1, 2). It belongs to the lipid transfer protein family that also includes LPS binding protein (LBP), cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Circulating levels of BPI are positively correlated with the levels of cholesterol, LDL cholesterol, and HDL cholesterol (3). Mature human BPI shares approximately 55% amino acid (aa) sequence identity with mouse and rat BPI. It can be seceted as a monomer or as a disulfide‑linked homodimer (4). It consists of a highly basic N‑terminal and a hydrophobic C‑terminal domain (5). Its N‑terminal domain confers the ability of BPI to bind bacterial lipopolysaccharide (LPS) found in the cell walls of Gram negative bacteria and to induce the lysis and phagocytosis of these bacteria (6‑9). It also blocks the endothelial cell response to endotoxin (10). BPI is stored in neutrophil and eosinophil granules for induced secretion during inflammation (11, 12). It is additionally expressed in mucosal epithelia and testis (10, 13). BPI can be retained on the surface of both neutrophils and epithelial cells, presumably by its hydrophobic C‑terminal domain (8, 10). BPI also functions as an anti-angiogenic molecule by inhibiting vascular endothelial cell proliferation and tubule formation (14). Like the antibacterial actions, this function is mediated by the N‑terminal region (15).

References

  1. Schultz, H. and J.P. Weiss (2007) Clin. Chim. Acta 384:12.
  2. Holweg, A. et al. (2011) Biochem. Soc. Trans. 39:1045.
  3. Esteve, E. et al. (2010) Thromb. Haemost. 103:780.
  4. Horwitz, A.H. et al. (1996) Protein Exp. Purif. 8:28.
  5. Gray, P.W. et al. (1989) J. Biol. Chem. 264:9505.
  6. Ooi, C.E. et al. (1987) J. Biol. Chem. 262:14891.
  7. Tobias, P.S. et al. (1997) J. Biol. Chem. 272:18682.
  8. Weersink, A.J. et al. (1993) J. Immunol. 150:253.
  9. Nishimura, H. et al. (2001) Immunology 103:519.
  10. Canny, G. et al. (2002) Proc. Natl. Acad. Sci. 99:3902.
  11. Weiss, J. and I. Olsson (1987) Blood 69:652.
  12. Calafat, J. et al. (1998) Blood 91:4770.
  13. Lennartsson, A. et al. (2005) J. Leukoc. Biol. 77:369.
  14. van der Schaft, D.W.J. et al. (2000) Blood 96:176.
  15. Rauniyar, R.K. et al. (2002) Invest. Ophthalmol. Vis. Sci. 43:503.

Long Name

Bactericidal/Permeability-increasing Protein

Alternate Names

BPIFD1, CAP 57, rBPI

Entrez Gene IDs

671 (Human); 329547 (Mouse); 296321 (Rat)

Gene Symbol

BPI

UniProt

P17213 (Human)

Additional BPI Products

Product Documents for Human BPI Antibody

Certificate of Analysis

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Product Specific Notices for Human BPI Antibody

For research use only

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  • Human BPI Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Human
    Verified Customer | Posted 11/02/2022
    Human BPI Antibody MAB74681

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