Human Brachyury NorthernLights™ NL557-conjugated Antibody

R&D Systems | Catalog # NL2085R

R&D Systems
Discontinued Product
NL2085R has been discontinued. View all Brachyury products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Polyclonal Goat IgG
View all Brachyury Primary Antibodies »

Product Specifications

Immunogen

E. coli‑derived, recombinant human Brachyury
Ser2-Glu202
Accession # O15178

Specificity

Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Brachyury NorthernLights™ NL557-conjugated Antibody

Detection of Brachyury in E9.5 mouse embryo sections.

Brachyury was detected in E9.5 mouse embryo sections using Human Brachyury NL557 conjugated Antibody (Catalog# NL2085R). Embryos were fixed in 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X 100, and 1% BSA. After blocking, sections were incubated with NL557 conjugated antibody at a final concentration of 1X (1:10 dilution) in blocking buffer for 3 hours at room temperature in the dark. Between each step, cells were washed with PBS containing BSA.

Applications for Human Brachyury NorthernLights™ NL557-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

1:10 dilution
Sample: E9.5 mouse embryo sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. See Certificate of Analysis for details.
*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Brachyury

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Entrez Gene IDs

6862 (Human)

Gene Symbol

TBXT

UniProt

O15178

Additional Brachyury Products

Product Documents for Human Brachyury NorthernLights™ NL557-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Brachyury NorthernLights™ NL557-conjugated Antibody

For research use only

Citations for Human Brachyury NorthernLights™ NL557-conjugated Antibody

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