Human Brachyury NorthernLights™ NL557-conjugated Antibody

Catalog #: NL2085R
1 Citation Datasheet / COA / SDS

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NL2085R

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Detection of Brachyury in E9.5 mouse embryo sections.

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Human Brachyury NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli‑derived, recombinant human Brachyury
Ser2-Glu202
Accession # O15178

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. See Certificate of Analysis for details.
*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Label

NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

1:10 dilution

E9.5 mouse embryo sections.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Brachyury in E9.5 mouse embryo sections. Brachyury was detected in E9.5 mouse embryo sections using Human Brachyury NL557 conjugated Antibody (Catalog# NL2085R). Embryos were fixed in 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X 100, and 1% BSA. After blocking, sections were incubated with NL557 conjugated antibody at a final concentration of 1X (1:10 dilution) in blocking buffer for 3 hours at room temperature in the dark. Between each step, cells were washed with PBS containing BSA.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Brachyury

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Entrez Gene IDs

6862 (Human)

Alternate Names

brachyury protein; Brachyury; MGC104817; Protein T; SAVA; T brachyury (mouse) homolog; T brachyury homolog; T Brachyury Transcription Factor; T, brachyury homolog (mouse); T-Box Transcription Factor T; TBXT; TF; TFT

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Brachyury NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1