Human BTN3A1/2/3 Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
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Human BTN3A1/2/3 Alexa Fluor® 647-conjugated Antibody in Data
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Human BTN3A1/2/3 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs.
Recombinant Monoclonal Mouse IgG1 Clone # 849203
Protein A or G purified from cell culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human CD277/BTN3A1
Accession # O00481
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD277/BTN3A1 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of BTN3A1/2/3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human BTN3A1/2/3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB7136R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BTN3A1/2/3

Butyrophilin 3A1 (BTN3A1), also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.

Long Name
Butyrophilin, Subfamily 3, Member A1/2/3
Alternate Names

Product Datasheets


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