Human BTN3A1/2/3 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB7136
MAB7136-SP
Human BTN3A1/2/3 Antibody in Data
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Human BTN3A1/2/3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 849203
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD277/BTN3A1
Met1-Gly254
Accession # O00481
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of BTN3A1/2/3 in Human Lymphocytes by Flow Cytometry. View Larger

Detection of BTN3A1/2/3 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human BTN3A1/2/3 Monoclonal Antibody (Catalog # MAB7136, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN3A1/2/3

Butyrophilin 3A1 (BTN3A1), also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.

Long Name
Butyrophilin, Subfamily 3, Member A1/2/3
Alternate Names
BTN3A1/2/3

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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