Human C-Reactive Protein/CRP QuicKit ELISA

Catalog # Availability Size / Price Qty
QK1707
Control Products Available
Human CRP Standard Curve ELISA
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Human C-Reactive Protein/CRP QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Plasma (10 uL)
Sensitivity
0.079 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, Plasma)
Specificity
Natural and recombinant human CRP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® QuicKit™ Human C-Reactive Protein/CRP Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human CRP levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CRP and antibodies raised against the recombinant protein. Results obtained using natural human CRP showed linear curves that were parallel to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human CRP.

Precision

Intra-Assay Precision (Precision within an assay) <div>Two samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean 3.75 26.1 4.03 24.8
Standard Deviation 0.146 1.08 0.195 1.31
CV% 3.9 4.1 4.8 5.3

Recovery

The recovery of human CRP spiked to three levels in samples throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 92-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human CRP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. 
Human CRP Linearity Curve

Data Examples

Human CRP Standard Curve ELISA

Human CRP QuicKit Spiked Recovery Competitor Comparison CRP is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Culture media recovery is 95% compared to 84% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Entrez Gene IDs:
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names:
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Assay Procedure

These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.

QuicKit ELISA assay procedure

FAQs

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