Human C1q R1/CD93 Quantikine ELISA Kit

  (1 citations)     
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Assay Procedure
Citations (1)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (25 uL), Urine (50 uL)
  • Sensitivity
    0.028 ng/mL
  • Assay Range
    0.1 - 5 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
  • Specificity
    Natural and recombinant human C1q R1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC55 , Quantikine Immunoassay Control Set 756 for Human C1q R1/CD93 - Please Inquire
Product Summary
The Quantikine Human C1q R1 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human C1q R1 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human C1q R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human C1q R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human C1q R1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.49 1.58 3.41 0.48 1.51 3.19
Standard Deviation 0.01 0.05 0.11 0.03 0.09 0.15
CV% 2 3.2 3.2 6.3 6 4.7

Recovery

The recovery of C1q R1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 99 92-108
EDTA Plasma (n=4) 104 94-118
Heparin Plasma (n=4) 102 91-115
Saliva (n=4) 104 94-112
Serum (n=4) 102 87-115
Urine (n=4) 97 93-102
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of C1q R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 C1q R1/CD93 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C1q R1/CD93
C1q R1 is also known as C1q Rp, collectin receptor, and AA4 antigen. C1q R1 mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL, and SP-A. It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets, and cells of myeloid origin. C1q R1 has also been identified as a hematopoietic stem cell marker.
  • Long Name:
    Complement Component C1q Receptor
  • Entrez Gene IDs:
    22918 (Human); 17064 (Mouse); 84398 (Rat)
  • Alternate Names:
    AA4 Antigen; C1q R1; C1q receptor 1; C1q Rp; C1q/MBL/SPA receptor; C1qR(P); C1qR1; C1qRp; CD93 antigenC1qR; CD93 molecule; CD93; CDw93C1QR1; Collectin Receptor; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Targeted proteomics reveals promising biomarkers of disease activity and organ involvement in antineutrophil cytoplasmic antibody-associated vasculitis
    Authors: J Ishizaki, A Takemori, K Suemori, T Matsumoto, Y Akita, KE Sada, Y Yuzawa, K Amano, Y Takasaki, M Harigai, Y Arimura, H Makino, M Yasukawa, N Takemori, H Hasegawa,
    Arthritis Res. Ther., 2017;19(1):218.
    Species: Human
    Sample Type: Serum

FAQs

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ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 756 for Human C1q R1/CD93

Ctrl QC55
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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