Human C1qR1/CD93 Quantikine ELISA Kit

R&D Systems | Catalog # DCD930

R&D Systems
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Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (25 µL), Urine (50 µL)

Sensitivity

0.028 ng/mL

Assay Range

0.078-5 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
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Product Summary for Human C1qR1/CD93 Quantikine ELISA Kit

The Quantikine Human C1q R1 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human C1q R1 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human C1q R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human C1q R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human C1q R1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human C1q R1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 0.49 1.58 3.41 0.48 1.51 3.19
Standard Deviation 0.01 0.05 0.11 0.03 0.09 0.15
CV% 2.0 3.2 3.2 6.3 6.0 4.7

Recovery for Human C1qR1/CD93 Quantikine ELISA Kit

The recovery of C1q R1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 99 92-108
EDTA Plasma (n=4) 104 94-118
Heparin Plasma (n=4) 102 91-115
Saliva (n=4) 104 94-112
Serum (n=4) 102 87-115
Urine (n=4) 97 93-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of C1q R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human C1q R1/CD93 ELISA Linearity

Scientific Data Images for Human C1qR1/CD93 Quantikine ELISA Kit

Human C1q R1/CD93 ELISA Standard Curve

Human C1q R1/CD93 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C1qR1/CD93

C1qR1 is also known as C1qRp, collectin receptor, and AA4 antigen. C1qR1 mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL, and SP-A. It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets, and cells of myeloid origin. C1qR1 has also been identified as a hematopoietic stem cell marker.

Long Name

Complement Component C1q Receptor

Alternate Names

AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Gene Symbol

CD93

Additional C1qR1/CD93 Products

Product Documents for Human C1qR1/CD93 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human C1qR1/CD93 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human C1qR1/CD93 Quantikine ELISA Kit

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  • Human C1qR1/CD93 Quantikine ELISA Kit Review
    Name: Md Imtiazul Islam
    Sample Tested: Urine
    Species: Human
    Verified Customer | Posted 01/13/2026
    CD93 ELISA
    We used this to detect CD93 from human urine and serum and it detect the molecule very well
    Human C1qR1/CD93 Quantikine ELISA Kit DCD930

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Protocols

View specific protocols for Human C1qR1/CD93 Quantikine ELISA Kit (DCD930):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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