Human C1qR1/CD93 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DCD930
Control Products Available
Human C1q R1/CD93 ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (1)
FAQs
Supplemental Products
Reviews

Human C1qR1/CD93 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (25 uL), Urine (50 uL)
Sensitivity
0.028 ng/mL
Assay Range
0.1 - 5 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human C1q R1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human C1q R1 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human C1q R1 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human C1q R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human C1q R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human C1q R1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.49 1.58 3.41 0.48 1.51 3.19
Standard Deviation 0.01 0.05 0.11 0.03 0.09 0.15
CV% 2 3.2 3.2 6.3 6 4.7

Recovery

The recovery of C1q R1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 99 92-108
EDTA Plasma (n=4) 104 94-118
Heparin Plasma (n=4) 102 91-115
Saliva (n=4) 104 94-112
Serum (n=4) 102 87-115
Urine (n=4) 97 93-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of C1q R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human C1q R1/CD93 ELISA Linearity

Data Examples

Human C1q R1/CD93 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C1qR1/CD93

C1q R1 is also known as C1q Rp, collectin receptor, and AA4 antigen. C1q R1 mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL, and SP-A. It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets, and cells of myeloid origin. C1q R1 has also been identified as a hematopoietic stem cell marker.

Long Name:
Complement Component C1q Receptor
Entrez Gene IDs:
22918 (Human); 17064 (Mouse); 84398 (Rat)
Alternate Names:
AA4 Antigen; C1q R1; C1q receptor 1; C1q Rp; C1q/MBL/SPA receptor; C1qR(P); C1qR1; C1qRp; CD93 antigenC1qR; CD93 molecule; CD93; CDw93C1QR1; Collectin Receptor; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citation for Human C1qR1/CD93 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Targeted proteomics reveals promising biomarkers of disease activity and organ involvement in antineutrophil cytoplasmic antibody-associated vasculitis
    Authors: J Ishizaki, A Takemori, K Suemori, T Matsumoto, Y Akita, KE Sada, Y Yuzawa, K Amano, Y Takasaki, M Harigai, Y Arimura, H Makino, M Yasukawa, N Takemori, H Hasegawa,
    Arthritis Res. Ther., 2017;19(1):218.
    Species: Human
    Sample Types: Serum

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

ELISA Controls

Supplemental ELISA Products

Reviews for Human C1qR1/CD93 Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Human C1qR1/CD93 Quantikine ELISA Kit and earn rewards!

Have you used Human C1qR1/CD93 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review