Human Calbindin D Quantikine ELISA Kit

    
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Assay Procedure
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Tissue Lysates (10 uL), Urine (25 uL)
  • Sensitivity
    0.015 ng/mL
  • Assay Range
    0.1 - 5 ng/mL
  • Specificity
    Natural and recombinant human Calbindin D.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC189 , Quantikine Immunoassay Control Set 855 for Human Calbindin D - Please Inquire
Product Summary
The Quantikine Human Calbindin D Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Calbindin D in tissue lysates and urine. It contains E. coli-expressed recombinant human Calbindin D and antibodies raised against the recombinant factor. Natural human Calbindin D showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural human Calbindin D.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Tissue Lysates, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.511 1.65 2.84 0.548 1.57 2.96
Standard Deviation 0.026 0.04 0.072 0.038 0.075 0.15
CV% 5.1 2.4 2.5 6.9 4.8 5.1

Recovery

The recovery of human Calbindin D spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Urine (n=4) 93 89-98
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Calbindin D were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
 Calbindin D/S100G [HRP]
Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Calbindin D

Calbindin D is a highly conserved vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin-D is widely expressed and regulates calcium homeostasis. Calbindin-D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium.

  • Long Name:
    Vitamin D-dependent Calcium-Binding Protein
  • Entrez Gene IDs:
    795 (Human); 12309 (Mouse); 24249 (Rat)
  • Alternate Names:
    CABP; CABP1; CABP28K; CABP9K; Calbindin 3; Calbindin D; Calbindin-D; Calbindin-D9K; S100G
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

FAQs

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ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 855 for Human Calbindin D

Ctrl QC189
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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