Detection of Human Calreticulin by Western Blot.|
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human adrenal gland tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB3898) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calreticulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Intracellular Staining by Flow Cytometry
|Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB3898, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
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Immunocytochemistry/Immunofluorescence: Human Calreticulin Antibody [MAB3898]
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