Human Calreticulin Antibody

MAB3898 has been discontinued and is replaced by AF3898MAB38981.
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Calreticulin in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 326203
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Calreticulin
    Met1-Asn180
    Accession # P27797
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Calreticulin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human adrenal gland tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB3898) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calreticulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Intracellular Staining by Flow Cytometry
Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB3898, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calreticulin
Human Calreticulin is a 55‑60 kDa, 400 amino acid, variably glycosylated intra- and extracellular Ca++-binding lectin that is ubiquitously expressed. It consists of three domains: a 180 aa N-terminal globular region, a 111 aa P-, or proline rich domain, and a 109 aa C-terminus. The 180 aa N-terminus (aa 18‑197) is termed Vasostatin. It is unclear if it is ever generated naturally via proteolytic processing. Vasostatin domain has many functions. It binds to RNA (aa 18‑27), has autocatalytic phosphorylase activity (aa 77‑197), binds to a KxFFKR motif on steroid hormone receptors, and serves as a lectin-type chaperone for ER-localized molecules. It also shows antiangiogenic activity, presumably by binding to laminin carbohydrates and blocking endothelial cell adhesion and proliferation. Human Calreticulin is 94% aa identical to mouse and rat Calreticulin.
  • Entrez Gene IDs:
    811 (Human); 12317 (Mouse); 64202 (Rat)
  • Alternate Names:
    Autoantigen Ro; CALR; Calregulin; Calreticulin; cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; ERp60; FLJ26680; grp60; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA; SSAHACBP; Vasostatin
Related Research Areas
Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Calreticulin Antibody MAB3898
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Excellent
 ICC/IF Human Anonymous 10/18/2016
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Immunocytochemistry/Immunofluorescence Human Calreticulin Antibody MAB3898
Immunocytochemistry/Immunofluorescence: Human Calreticulin Antibody [MAB3898]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedmesenchymal cells
SpeciesHuman

Order Details

    MAB3898 has been replaced by
    AF3898
    MAB38981

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