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Human Carbonic Anhydrase VA/VB (CA5A/5B) Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB5388G Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5388G-100UG

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Human Carbonic Anhydrase VA/VB (CA5A/5B) Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase VA/VB (CA5A/5B) in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6‑14 is observed.
Source
Monoclonal Mouse IgG2b Clone # 487908
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase VA/VB (CA5A/5B)
Ala40-Ser305
Accession # P35218
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbonic Anhydrase VA/CA5A

Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O → HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA5A and CA5B are homologous proteins that are encoded by different genes locating in different chromosomes. However, they both are mitochondrial proteins and have low Carbonic Anhydrase activity. The amino acid sequence of human CA5A is 98%, 80% and 76% identical to that of chimpanzee, dog and mouse.

Entrez Gene IDs
763 (Human); 54233 (Rat)
Alternate Names
CA5A; CA5carbonic anhydrase 5A, mitochondrial; Carbonate dehydratase VA; carbonic anhydrase V, mitochondrial; Carbonic Anhydrase VA; carbonic anhydrase VA, mitochondrial; carbonic dehydratase; CAV; CAVA; CA-VA; EC 4.2.1.1

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COA

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