Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O → HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA5A and CA5B are homologous proteins that are encoded by different genes locating in different chromosomes. However, they both are mitochondrial proteins and have low Carbonic Anhydrase activity. The amino acid sequence of human CA5A is 98%, 80% and 76% identical to that of chimpanzee, dog and mouse.
Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
R&D Systems | Catalog # MAB5388
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 487908
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Product Specifications
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase VA/VB (CA5A/5B)
Ala40-Ser305
Accession # P35218
Ala40-Ser305
Accession # P35218
Specificity
Detects human Carbonic Anhydrase VA/VB (CA5A/5B) in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6‑14 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
Carbonic Anhydrase VA/VB (CA5A/5B) in Human Liver.
Carbonic Anhydrase VA/VB (CA5A/5B) was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Carbonic Anhydrase VA/ VB (CA5A/5B) Monoclonal Antibody (Catalog # MAB5388) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Applications for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase VA (Catalog # 3049-CA)
Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA)
Sample: Recombinant Human Carbonic Anhydrase VA (Catalog # 3049-CA)
Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Carbonic Anhydrase VA/VB (CA5A/5B)
Alternate Names
CA5;CA5AD;Carbonic anhydrase 5A, mitochondrial;CAV;CAVA;GS1-21A4.1
Gene Symbol
CA5A
UniProt
Additional Carbonic Anhydrase VA/VB (CA5A/5B) Products
Product Documents for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
Product Specific Notices for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars