Human CCL2/JE/MCP-1 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9,10, MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or recombinant rat CCL20.
  • Source
    Monoclonal Mouse IgG1 Clone # 24822
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL2/JE/MCP-1
    Accession # P13500
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of CCL2/JE/MCP‑1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 250 ng/mL LPS overnight were stained with Mouse Anti-Human CCL2/JE/MCP‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC279G) and Mouse Anti-Human CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB1777A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL2/JE/MCP-1
CCL2 is a beta -chemokine that is also known as Monocyte Chemotactic Protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes and basophils. MCP-1 exists in equilibrium as either a monomer or homodimer, and forms a heterodimer with multiple chemokines. Human and mouse CCL2 share 58% amino acid sequence identity.
  • Entrez Gene IDs:
    6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
  • Alternate Names:
    MCAF; MCP-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

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