Detection of CCL2/JE/MCP‑1 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with 250 ng/mL LPS overnight were stained with Mouse Anti-Human CCL2/JE/MCP‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC279G) and Mouse Anti-Human CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB1777A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCL2 is a beta -chemokine that is also known as Monocyte Chemotactic Protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes and basophils. MCP-1 exists in equilibrium as either a monomer or homodimer, and forms a heterodimer with multiple chemokines. Human and mouse CCL2 share 58% amino acid sequence identity.
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