Human CCL26/Eotaxin-3 Quantikine ELISA Kit

R&D Systems | Catalog # DCC260B

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

0.215 pg/mL

Assay Range

3.13-200 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, Plasma)
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Product Summary for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

The Quantikine® Human CCL26/Eotaxin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Eotaxin-3 levels in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed, recombinant human Eotaxin-3 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human Eotaxin-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin-3.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Species

Human

Specificity

Natural and recombinant human Eotaxin-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 43.6 86.0 131 44.4 91.7 139
Standard Deviation 0.570 1.06 1.77 3.66 7.79 11.6
CV% 1.3 1.2 1.4 8.2 8.5 8.3

Recovery for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

The recovery of human Eotaxin-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 102 92-119
Cell Lysis Buffer (n=1) 97 94-99
EDTA Plasma (n=5) 94 84-105
Heparin Plasma (n=5) 95 86-109
Serum (n=5) 93 81-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Eotaxin-3 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human Eotaxin-3 ELISA Linearity

Scientific Data Images for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

Human Eotaxin-3 ELISA Standard Curve

Human Eotaxin-3 ELISA Standard Curve

Human Eotaxin-3 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL26/Eotaxin-3

CCL26, also known as Eotaxin-3 and SCYA26, is a small cytokine that is a member of the CC chemokine family. It is only 94 amino acids (aa) in length, with aa 1-23 serving as a putative signal peptide. Human CCL26/Eotaxin-3 has a predicted molecular weight of approximately 11 kDa, and the mature protein only shares 27% aa sequence identity with the mouse ortholog. CCL26/Eotaxin-3 is constitutively expressed at low levels in various tissues including in the heart and ovary. Its expression in upregulated in vascular endothelial cells by IL-13 and IL-4. CCL26/Eotaxin-3 is a selective agonist for CCR3, as serves as a chemoattractant for cells that express CCR3, such as eosinophils, basophils, and Th2 cells. It has also been shown to act as an antagonist for the receptors CCR1 and CCR5. CCL26/Eotaxin-3 plays a role in allergic reactions.

Alternate Names

Eotaxin-3, Eotaxin3, SCYA26

Entrez Gene IDs

10344 (Human)

Gene Symbol

CCL26

Additional CCL26/Eotaxin-3 Products

Product Documents for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

For research use only

Citations for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

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  • Human CCL26/Eotaxin-3 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell Extracts
    Verified Customer | Posted 05/22/2025
    Increased sensitivity compared to DuoSet, protein extract from cells used neat
    Human CCL26/Eotaxin-3 Quantikine ELISA Kit DCC260B

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