Human CCL26/Eotaxin-3 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DCC260B
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Human Eotaxin-3 Simple Plex Assay Linearity
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Product Details
Citations (14)
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Human CCL26/Eotaxin-3 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.215 pg/mL
Assay Range
3.1 - 200 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, Plasma)
Specificity
Natural and recombinant human Eotaxin-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human CCL26/Eotaxin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Eotaxin-3 levels in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed, recombinant human Eotaxin-3 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human Eotaxin-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 43.6 86 131 44.4 91.7 139
Standard Deviation 0.57 1.06 1.77 3.66 7.79 11.6
CV% 1.3 1.2 1.4 8.2 8.5 8.3

Recovery

The recovery of human Eotaxin-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 102 92-119
Cell Lysis Buffer (n=1) 97 94-99
EDTA Plasma (n=5) 94 84-105
Heparin Plasma (n=5) 95 86-109
Serum (n=5) 93 81-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Eotaxin-3 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Eotaxin-3 ELISA Linearity

Data Examples

Human Eotaxin-3 ELISA Standard Curve

Human Eotaxin-3 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
    Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
    Br J Haematol, 2014;164(2):286-95.
    Species: Human
    Sample Types: Plasma
  2. Leukotriene D4 and interleukin-13 cooperate to increase the release of eotaxin-3 by airway epithelial cells.
    Authors: Provost V, Langlois A, Chouinard F, Rola-Pleszczynski M, Chakir J, Flamand N, Laviolette M
    PLoS ONE, 2012;7(8):e43544.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. High Levels of CCL26 in Blister Fluid and Sera of Patients with Bullous Pemphigoid.
    Authors: Kagami S, Kai H, Kakinuma T
    J. Invest. Dermatol., 2012;132(1):249-51.
    Species: Human
    Sample Types: Serum
  4. Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Fujita H
    J. Invest. Dermatol., 2010;130(9):2304-11.
    Species: Human
    Sample Types: Serum
  5. Post-transcriptional silencing of CCR3 downregulates IL-4 stimulated release of eotaxin-3 (CCL26) and other CCR3 ligands in alveolar type II cells.
    Authors: Taka E, Errahali YJ, Abonyo BO, Bauer DM, Heiman AS
    Cytokine, 2008;44(3):342-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007;120(5):1118-25.
    Species: Human
    Sample Types: BALF
  7. Systemic responses after bronchial aspirin challenge in sensitive patients with asthma.
    Authors: Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML
    J. Allergy Clin. Immunol., 2007;121(2):348-54.
    Species: Human
    Sample Types: Serum
  8. Eotaxin-3 and a uniquely conserved gene-expression profile in eosinophilic esophagitis.
    Authors: Assa&amp;amp;apos;ad AH
    J. Clin. Invest., 2006;116(2):536-47.
    Species: Human
    Sample Types: Plasma
  9. Anti-IL-5 (mepolizumab) therapy for eosinophilic esophagitis.
    Authors: Stein ML, Collins MH, Villanueva JM, Kushner JP, Putnam PE, Buckmeier BK, Filipovich AH, Assa&amp;amp;apos;ad AH, Rothenberg ME
    J. Allergy Clin. Immunol., 2006;118(6):1312-9.
    Species: Human
    Sample Types: Plasma
  10. Markers of eosinophilic inflammation and risk prediction in patients with coronary artery disease.
    Authors: Falcone C, Minoretti P, D&amp;amp;apos;Angelo A, Buzzi MP, Coen E, Emanuele E, Aldeghi A, Olivieri V, Geroldi D
    Eur. J. Clin. Invest., 2006;36(4):211-7.
    Species: Human
    Sample Types: Plasma
  11. Membrane-bound eotaxin-3 mediates eosinophil transepithelial migration in IL-4-stimulated epithelial cells.
    Authors: Yuan Q, Campanella GS, Colvin RA, Hamilos DL, Jones KJ, Mathew A, Means TK, Luster AD
    Eur. J. Immunol., 2006;36(10):2700-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Regulatory mechanisms of galectin-9 and eotaxin-3 synthesis in epidermal keratinocytes: possible involvement of galectin-9 in dermal eosinophilia of Th1-polarized skin inflammation.
    Authors: Igawa K, Satoh T, Hirashima M, Yokozeki H
    Allergy, 2006;61(12):1385-91.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2006;36(5):237-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Eotaxin-3 and interleukin-5 pleural fluid levels are associated with pleural fluid eosinophilia in post-coronary artery bypass grafting pleural effusions.
    Authors: Kalomenidis I, Stathopoulos GT, Barnette R, Guo Y, Peebles RS, Blackwell TS, Light RW
    Chest, 2005;127(6):2094-100.
    Species: Human
    Sample Types: Pleural Effusion

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