Human CCL8/MCP‑2 Antibody

R&D Systems | Catalog # AB-281-NA

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL8/MCP-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL8/MCP-2
Gln24-Pro99
Accession # P80075

Specificity

Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) MCP-1 and rhMCP-3 is observed. Neutralizes the biological activity of recombinant human MCP-2, but will not neutralize the biological activity of MCP-1 or MCP-3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL8/MCP‑2 Antibody

Chemotaxis Induced by CCL8/MCP‑2 and Neutral-ization by Human CCL8/ MCP‑2 Antibody.

Chemotaxis Induced by CCL8/MCP‑2 and Neutral-ization by Human CCL8/ MCP‑2 Antibody.

Recombinant Human CCL8/MCP-2 (Catalog # 281-CP) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL8/MCP-2 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL8/MCP-2 Polyclonal Antibody (Catalog # AB-281-NA). The ND50 is typically 60-120 µg/mL.

Applications for Human CCL8/MCP‑2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP)

Neutralization

Measured by its ability to neutralize CCL8/MCP‑2-induced chemotaxis in human monocytes. The Neutralization Dose (ND50) is typically 60-120 µg/mL in the presence of 0.4 µg/mL Recombinant Human CCL8/MCP‑2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL8/MCP-2

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MCP-2, MCP2

Entrez Gene IDs

6355 (Human); 20307 (Mouse)

Gene Symbol

CCL8

UniProt

P80075

Additional CCL8/MCP-2 Products

Product Documents for Human CCL8/MCP‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL8/MCP‑2 Antibody

For research use only

Citations for Human CCL8/MCP‑2 Antibody

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