Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/|
MCP‑2 Antibody. Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL8/MCP‑2 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL8/MCP‑2 Polyclonal Antibody (Catalog # AB-281-NA). The ND50 is typically 60‑120 µg/mL.
MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.
Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
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