Human CD15/Lewis X Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD15/Lewis X in direct ELISAs.
  • Source
    Monoclonal Mouse IgM Clone # ICRF29-2
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Human granulocytes
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD15/Lewis X in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CD15/Lewis X Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7368G, filled histogram) or isotype control antibody (Catalog # IC015G, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD15/Lewis X
Lewis X (Lex) is a glycan structure (Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta -) that forms the terminus of N-linked carbohydrates such as the antigenic epitope Stage-Specific Embryonic Antigen-1 (SSEA-1/CD15). It is widely expressed, can be found on glycoproteins, glycolipids and proteoglycans and is a blood group antigen. Lex is a marker for mouse embryonic stem and cancer cells, and is expressed on leukocytes, germ cells and neuronal stem cells in both mouse and human. Lex is thought to enhance cell adhesion, either directly or by promoting integrin activation.
  • Alternate Names:
    CD 15; CD15; CD-15; Lewis X; Lewis-X; LeX; SSEA-1; stage-specific embryonic antigen 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Mouse IgM Alexa Fluor® 488-conjugated Control

Ctrl IC015G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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