Detection of CD15/Lewis X in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Mouse Anti‑Human CD15/Lewis X Monoclonal Antibody (Catalog # MAB7368, filled histogram) or Mouse IgM control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD15/Lewis X
Lewis X (Lex)
is a glycan structure (Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta -) that forms the terminus of N-linked
carbohydrates such as the antigenic epitope Stage-Specific Embryonic Antigen-1
(SSEA-1/CD15). It is widely expressed, can be found on glycoproteins,
glycolipids and proteoglycans and is a blood group antigen. Lex is a
marker for mouse embryonic stem and cancer cells, and is expressed on
leukocytes, germ cells and neuronal stem cells in both mouse and human. Lex
is thought to enhance cell adhesion, either directly or by promoting integrin
CD 15; CD15; CD-15; Lewis X; Lewis-X; LeX; SSEA-1; stage-specific embryonic antigen 1
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