Human CD15/Lewis X Antibody

Catalog # Availability Size / Price Qty
MAB7368
MAB7368-SP

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Detection of CD15/Lewis X in Human Blood Granulocytes by Flow Cytometry.
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Citations (1)
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Human CD15/Lewis X Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD15/Lewis X in direct ELISAs.
Source
Monoclonal Mouse IgM Clone # ICRF29-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
human granulocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD15/Lewis X antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of CD15/Lewis X in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CD15/Lewis X Monoclonal Antibody (Catalog # MAB7368, filled histogram) or Mouse IgM control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).

Immunohistochemistry CD15/Lewis X antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

CD15/Lewis X in Human Breast Cancer Tissue. CD15/Lewis X was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue (left panel; positive staining) and normal breast (right panel; negative staining) using Mouse Anti-Human CD15/Lewis X Monoclonal Antibody (Catalog # MAB7368) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD15/Lewis X

Lewis X (Lex) is a glycan structure (Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta -) that forms the terminus of N-linked carbohydrates such as the antigenic epitope Stage-Specific Embryonic Antigen-1 (SSEA-1/CD15). It is widely expressed, can be found on glycoproteins, glycolipids and proteoglycans and is a blood group antigen. Lex is a marker for mouse embryonic stem and cancer cells, and is expressed on leukocytes, germ cells and neuronal stem cells in both mouse and human. Lex is thought to enhance cell adhesion, either directly or by promoting integrin activation.

Alternate Names
CD 15; CD15; CD-15; Lewis X; Lewis-X; LeX; SSEA-1; stage-specific embryonic antigen 1

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Citation for Human CD15/Lewis X Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. HIF1alpha/HIF2alpha induces glioma cell dedifferentiation into cancer stem cells through Sox2 under hypoxic conditions
    Authors: P Wang, S Gong, B Liao, J Pan, J Wang, D Zou, L Zhao, S Xiong, Y Deng, Q Yan, N Wu
    Journal of Cancer, 2022;13(1):1-14.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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