Human CD15/Lewis X Alexa Fluor® 750-conjugated Antibody

FAB7368S Datasheet / COA / SDS

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FAB7368S-100UG

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Human CD15/Lewis X Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD15/Lewis X in direct ELISAs.

Source

Monoclonal Mouse IgM Clone # ICRF29-2

Immunogen

human granulocytes

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

Human peripheral blood granulocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD15/Lewis X

Lewis X (Le^x) is a glycan structure (Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta -) that forms the terminus of N-linked carbohydrates such as the antigenic epitope Stage-Specific Embryonic Antigen-1 (SSEA-1/CD15). It is widely expressed, can be found on glycoproteins, glycolipids and proteoglycans and is a blood group antigen. Le^x is a marker for mouse embryonic stem and cancer cells, and is expressed on leukocytes, germ cells and neuronal stem cells in both mouse and human. Le^x is thought to enhance cell adhesion, either directly or by promoting integrin activation.

Alternate Names

CD 15; CD15; CD-15; Lewis X; Lewis-X; LeX; SSEA-1; stage-specific embryonic antigen 1