Human CD1b Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CD1b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1c, rhCD1d, rhCD1e, recombinant mouse (rm) CD1d1, or rmCD1d2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 737249
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD1b
    Accession # P29016
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD1b in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1b Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7446G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD1b
CD1b is a 44-46 kDa member of the CD1 family of proteins. It is one of five CD1 isoforms (CD1a-e) that resemble MHC-I type molecules. Based on structure, CD1a, 1b and 1c can be grouped together, with CD1d and 1e existing as stand-alone forms. CD1b is a 316 amino acid (aa) type I transmembrane glycoprotein that contains one Ig-like domain in its ECD. It is expressed in the late endosome/lysosome compartment of myeloid-type dendritic cells. Following synthesis, CD1b associates with SAP-C, binds two short lipids in an unusually large ligand-binding groove, and transits either to the plasma membrane, or more commonly, the endosomal compartment. In either case, exposure to Gm- lipid A or mycobacterial glyolipid results in a substitution of the foreign lipid for the two short-chain self lipids, followed by its presentation on the plasma membrane. Select alpha beta and gamma δ T cell recognize these lipids and typically repond with Th1-type cytokines (IFN-gamma and TNF-alpha ). Unlike other CD1 family molecules, CD1b does not seem to have a soluble form. There is no rodent counterpart to human CD1b, only to CD1d.
  • Entrez Gene IDs:
    910 (Human)
  • Alternate Names:
    CD1; CD1A; CD1b antigen; CD1B antigen, b polypeptide; CD1b molecule; CD1b; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3; MGC125990; MGC125991; R1; T-cell surface glycoprotein CD1b
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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