Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 737249
View all CD1b Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD1b
Ser18-Ser303
Accession # P29016

Specificity

Detects human CD1b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1c, rhCD1d, rhCD1e, recombinant mouse (rm) CD1d1, or rmCD1d2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD1b Antibody

Detection of CD1b antibody in MOLT-4 Human Cell Line antibody by Flow Cytometry.

Detection of CD1b in MOLT‑4 Human Cell Line by Flow Cytometry.

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1b Monoclonal Antibody (Catalog # MAB7446, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human CD1b Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD1b

CD1b is an approximately 40-50 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1b associates with beta 2-microglobulin in endosomes and the Golgi where it facilitates the processing of glycolipids for their presentation by other CD1 family proteins. CD1b is expressed by most nonhuman mammals but not by mice or rats. It contains one Ig-like domain in its extracellular region. Alternate splicing of human CD1b generates an additional isoform that lacks 55 amino acids of the Ig-like domain.

Alternate Names

CD1b

Entrez Gene IDs

910 (Human)

Gene Symbol

CD1B

UniProt

P29016

Additional CD1b Products

Product Documents for Human CD1b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD1b Antibody

For research use only

Related Research Areas

T Cell Antigen Recognition

Citations for Human CD1b Antibody

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