Human CD200 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB27241R-100UG
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Human CD200 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD200 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD200 is observed.
Source
Monoclonal Mouse IgG1 Clone # 325516
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD200
Gln31-Gly232
Accession # P41217.3
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (3). A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5‑7). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (8). CD200 and CD200R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact (6, 7, 10). In T cells, however, CD200 functions as a co‑stimulatory molecule independent of the CD28 pathway (11). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14‑16).

References
  1. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  2. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  3. McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
  4. Wright, G.J. et al. (2001) Immunology 102:173.
  5. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  7. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  8. Hoek, R.M. et al. (2000) Science 290:1768.
  9. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  10. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  11. Borriello, F. et al. (1997) J. Immunol. 158:4548.
  12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  13. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  14. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
  15. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
  16. Langlais, C.L. et al. (2006) J. Virol. 80:3098.
Entrez Gene IDs
4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2

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