Human CD200R1 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3414F

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Detection of CD200 R1 in Human Blood Monocytes by Flow Cytometry.
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Citations (2)
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Human CD200R1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD200 R1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 380525
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD200 R1
Ala27-Leu266 (predicted)
Accession # NP_620161
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD200 R1 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CD200 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD200 R1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3414F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily (1‑3). The standard human CD200 R1 cDNA encodes a 325 amino acid (aa) precursor that includes a 28 aa signal sequence, a 215 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, human CD200 R1 shares 56% aa sequence identity with mouse and rat CD200 R1. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted. The protein expressed here contains a mature region that is identical to that of the standard form. There is an N-terminal extension of 25 aa that, in the standard form, is part of the signal sequence. In human, a separate CD200 R12 gene encodes a protein that shares 81% ECD aa identity with CD200 R11. In mouse, at least four genes for CD200 R1-like molecules have been described (4‑6). CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (7‑9), while its ligand, CD200, is widely distributed (10). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (11). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (12). The capacity of CD200 R1-like molecules to interact with CD200 is controversial (5, 13). CD200 R1 propagates inhibitory signals despite its lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (8, 9, 14, 15) CD200 R1-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 6).

References
  1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
  2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  4. Wright, G.J. et al. (2003) J. Immunol. 171:3034.
  5. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  6. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
  7. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  8. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  9. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  10. Wright, G.J. et al. (2001) Immunology 102:173.
  11. Hoek, R.M. et al. (2000) Science 290:1768.
  12. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  13. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  14. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  15. Zhang, S. et al. (2004) J. Immunol. 173:6786.
Long Name
CD200 Receptor 1
Entrez Gene IDs
131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)
Alternate Names
CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor

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Citations for Human CD200R1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CD200/CD200 receptor axis in psoriasis vulgaris
    Authors: AA Ismail, HM Donia, HM Ghatesh, CI Farid
    PLoS ONE, 2020;15(3):e0230621.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Soluble CD200 is critical to engraft chronic lymphocytic leukemia cells in immunocompromised mice.
    Authors: Wong K, Brenneman F, Chesney A, Spaner D, Gorczynski R
    Cancer Res, 2012;72(19):4931-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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