Detection of CD277/BTN3A1 in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD277/BTN3A1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB7136R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Butyrophilin 3A1 (BTN3A1), also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.
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