CD47, also known as Integrin-associated Protein (IAP), is a ubiquitous 50 kDa multipass transmembrane protein with a single V-type Ig-like domain at its N-terminus. CD47 binding to SIRP alpha prevents the phagocytic engulfment of viable cells. It also binds to VEGFR2, inducing VEGFR2 signaling in both T cells and endothelial cells. Thrombospondin interaction with CD47 on T cells reduces T cell proliferation and inflammatory reactions. Alternative splicing generates isoforms with truncated cytoplasmic domains. Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47.
Human CD47 APC‑conjugated Antibody
R&D Systems | Catalog # FAB4670A
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 472603
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD47
Gln19-Pro139
Accession # Q08722
Gln19-Pro139
Accession # Q08722
Specificity
Detects human CD47 in flow cytometry. Stains human CD47 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD47 APC‑conjugated Antibody
Detection of CD47 in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 APC-conjugated Monoclonal Antibody (Catalog # FAB4670A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD47 APC‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: CD47
Alternate Names
CD47, IAP, MER6, OA3
Entrez Gene IDs
Gene Symbol
CD47
UniProt
Additional CD47 Products
Product Documents for Human CD47 APC‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CD47 APC‑conjugated Antibody
For research use only
Citations for Human CD47 APC‑conjugated Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
