In a functional flow cytometry test, 2.5 μg/mL of Mouse anti-HumanCD47 Antibody (Catalog #MAB4670) will block the binding of 100 ng/mLRecombinant Human SIRP alpha /CD172a Fc Chimera (Catalog # 4546-SA)HEK293 humanembryonic kidney cell line transfected with recombinant human CD47.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of CD47 in Human Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 Monoclonal Antibody (Catalog # MAB4670, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
SIRP alpha /CD172aBinding toCD47-transfected HEK293 HumanCell Line is Blocked byHuman CD47 Antibody.
In afunctional flowcytometry test,biotinylated recombinant Human SIRP alpha /CD172a (100ng/mL, Catalog #4546-SA) binds to Human CD47-transfected HEK293 human embryonic kidney cell line (black dotted line). Binding is completely blocked (orange histogram) by 2.5 μg/mL of Mouse Anti-Human CD47 MonoclonalAntibody (Catalog # MAB4670). Mouse IgG1 isotype (Catalog #MAB002) at 2.5 μg/mL was used as a control (blue line).Cells were stained with Streptavidin-APC (Catalog # F0050).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD47, also known as integrin-associated protein, is a ubiquitous 50 kDa multipass transmembrane protein with a single IgV-like domain at its N-terminus. CD47 binding to SIRP alpha prevents the phagocytic engulfment of viable cells. Thrombospondin interaction with CD47 on T cells reduces T cell proliferation and inflammatory reactions. Alternate splicing generates isoforms with truncated cytoplasmic domains. Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47.
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