Human CD47 Antibody

Catalog # Availability Size / Price Qty
MAB4670
MAB4670-SP
Detection of CD47 in Human Lymphocytes by Flow Cytometry.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human CD47 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD47. Stains human CD47 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 472603
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD47
Gln19-Pro139
Accession # Q08722
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional flow cytometry test, 2.5 μg/mL of Mouse anti-HumanCD47 Antibody (Catalog #MAB4670) will block the binding of 100 ng/mLRecombinant Human SIRP alpha /CD172a Fc Chimera (Catalog # 4546-SA)HEK293 humanembryonic kidney cell line transfected with recombinant human CD47.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD47 antibody in Human Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD47 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 Monoclonal Antibody (Catalog # MAB4670, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Blockade of Receptor-ligand Interaction SIRP alpha /CD172a Binding to CD47-transfected HEK293 Human Cell Line is Blocked by Human CD47 Antibody. View Larger

SIRP alpha /CD172a Binding to CD47-transfected HEK293 Human Cell Line is Blocked by Human CD47 Antibody. In a functional flow cytometry test, biotinylated recombinant Human SIRPa/CD172a (100 ng/mL, Catalog # 4546-SA) binds to Human CD47-transfected HEK293 human embryonic kidney cell line (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human CD47 Monoclonal Antibody (Catalog # MAB4670). Mouse IgG1isotype (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD47

CD47, also known as integrin-associated protein, is a ubiquitous 50 kDa multipass transmembrane protein with a single IgV-like domain at its N-terminus. CD47 binding to SIRP alpha prevents the phagocytic engulfment of viable cells. Thrombospondin interaction with CD47 on T cells reduces T cell proliferation and inflammatory reactions. Alternate splicing generates isoforms with truncated cytoplasmic domains. Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47.

Entrez Gene IDs
961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen

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Citation for Human CD47 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CD47 as a potential prognostic marker for oral leukoplakia and oral squamous cell carcinoma
    Authors: X Ye, X Wang, R Lu, J Zhang, X Chen, G Zhou
    Oncol Lett, 2018;15(6):9075-9080.

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CD47 Antibody

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Human CD47 Antibody
By Anonymous on 07/02/2022
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

As expected.


Human CD47 Antibody
By Anonymous on 10/07/2018
Application: ICC/IF Sample Tested: 2120Ep human embryonal carcinoma cell line,Oral squamous cell carcinoma,The oral cancer cells Tca8113 Species: Human