Human CD68/SR‑D1 APC‑conjugated Antibody

R&D Systems | Catalog # IC20401A

R&D Systems
Discontinued Product
IC20401A has been discontinued. View all CD68/SR-D1 products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 298807
View all CD68/SR-D1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
Asn22-Ile320
Accession # AAB25811

Specificity

Detects human CD68/SR‑D1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD68/SR‑D1 APC‑conjugated Antibody

Detection of CD68/SR‑D1 in Human Macrophages by Flow Cytometry.

Human CD14+ monocytes were selected from PBMCs using MagCellect Human CD14+ Cell Isolation Kit (MAGH105) and cultured for four days in recombinant human M-CSF (216-MC; 10 ng/mL) then stained with (A) Mouse Anti-Human CD68/SR-D1 APC-conjugated Monoclonal Antibody (Catalog # IC20401A) or (B) isotype control antibody (IC0041A) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Detection of CD68/SR-D1 antibody in U937 Human Histiocytic Lymphoma Cell Line antibody by Flow Cytometry.

Detection of CD68/SR‑D1 in U937 Human Histiocytic Lymphoma Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CD68/SR-D1 APC-conjugated Monoclonal Antibody (Catalog # IC20401A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.

Applications for Human CD68/SR‑D1 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human CD14+ monocytes cultured for four days in recombinant human M-CSF (Catalog # 216-MC; 10 ng/mL) and U937 human histiocytic lymphoma cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Gene Symbol

CD68

UniProt

AAB25811

Additional CD68/SR-D1 Products

Product Documents for Human CD68/SR‑D1 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human CD68/SR‑D1 APC‑conjugated Antibody

For research use only

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