Recombinant Human M-CSF Protein 216-MC-005: R&D Systems

Recombinant Human M-CSF Protein

(117 citations)
(6 Reviews)
  
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5-1.5 ng/mL.
    The specific activity of Recombinant Human M-CSF is approximately 1.68 x 105 IU/μg, which is calibrated against recombinant human
    M-CSF WHO International Standard (NIBSC code: 89/512).
  • Source
    E. coli-derived Glu33-Ser190, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    18.5 kDa (monomer)
  • SDS-PAGE
    37 kDa, non-reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
216-MC
 
216-MC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 50-500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 50-500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human M-CSF (Catalog # 216-MC) stimulates cell proliferation of the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner. The ED50 for this effect is 0.5-1.5 ng/mL.
1 μg/lane of Recombinant Human M-CSF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 19 kDa and 35 kDa, respectively.
Background: M-CSF
M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode
M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N‑terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M‑CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.
  • References:
    1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
    2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
    3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
    4. Ryan, G.R. et al. (2001) Blood 98:74.
    5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
    6. Nandi, S. et al. (2006) Blood 107:786.
    7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
    8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
    9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
    10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
    11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
    12. Kawasaki, E.S. et al. (1985) Science 230: 291.
    13. Wong, G.G. et al. (1987) Science 235:1504.
  • Long Name:
    Macrophage Colony Stimulating Factor
  • Entrez Gene IDs:
    1435 (Human); 12977 (Mouse)
  • Alternate Names:
    colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

117 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
    Authors: KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis
    Nat. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. CLEC5A is a critical receptor in innate immunity against Listeria infection
    Authors: ST Chen, FJ Li, TY Hsu, SM Liang, YC Yeh, WY Liao, TY Chou, NJ Chen, M Hsiao, WB Yang, SL Hsieh
    Nat Commun, 2017;8(1):299.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Casein Kinase 2 Interacting Protein-1 regulates M1 and M2 inflammatory macrophage polarization
    Authors: Y Chen, W Liu, Y Wang, L Zhang, J Wei, X Zhang, F He, L Zhang
    Cell. Signal, 2017;33(0):107-121.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Siglec-1 initiates formation of the virus-containing compartment and enhances macrophage-to-T cell transmission of HIV-1
    Authors: JE Hammonds, N Beeman, L Ding, S Takushi, AC Francis, JJ Wang, GB Melikyan, P Spearman
    PLoS Pathog, 2017;13(1):e1006181.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Mycobacterium tuberculosis ESAT6 and CPF10 Induce Adenosine Deaminase 2 mRNA Expression in Monocyte-Derived Macrophages
    Authors: MJ Bae, S Ryu, HJ Kim, SI Cha, CH Kim, J Lee
    Tuberc Respir Dis (Seoul), 2017;80(1):77-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Time-lapse reveals that osteoclasts can move across the bone surface while resorbing
    Authors: K Søe, JM Delaissé
    J. Cell. Sci., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Structure of Nascent Chromatin Is Essential for Hematopoietic Lineage Specification
    Authors: S Petruk, SA Mariani, M De Dominic, P Porazzi, V Minieri, J Cai, L Iacovitti, N Flomenberg, B Calabretta, A Mazo
    Cell Rep, 2017;19(2):295-306.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. HIV and HIV-Tat inhibit LPS-induced IL-27 production in human macrophages by distinct intracellular signaling pathways
    Authors: N Gajanayaka, S O'Hara, Y Konarski, J Fernandes, K Muthumani, M Kozlowski, JB Angel, A Kumar
    J. Leukoc. Biol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Chemokine signals are crucial for enhanced homing and differentiation of circulating osteoclast progenitor cells
    Authors: A Sucur, Z Jajic, M Artukovic, MI Matijasevi, B Anic, D Flegar, A Markotic, T Kelava, S Ivcevic, N Kovacic, V Katavic, D Grcevic
    Arthritis Res. Ther., 2017;19(1):142.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Regeneration of long-distance peripheral nerve defects after delayed reconstruction in healthy and diabetic rats is supported by immunomodulatory chitosan nerve guides
    Authors: L Stenberg, M Stö beta el, G Ronchi, S Geuna, Y Yin, S Mommert, L Mårtensson, J Metzen, C Grothe, LB Dahlin, K Haastert-T
    BMC Neurosci, 2017;18(1):53.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Semen amyloids participate in spermatozoa selection and clearance
    Authors: NR Roan, N Sandi-Monr, N Kohgadai, SM Usmani, KG Hamil, J Neidleman, M Montano, L Ständker, A Röcker, M Cavrois, J Rosen, K Marson, JF Smith, CD Pilcher, F Gagsteiger, O Sakk, M O'Rand, PV Lishko, F Kirchhoff, J Münch, WC Greene
    Elife, 2017;6(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. SNX10 gene mutation leading to osteopetrosis with dysfunctional osteoclasts
    Authors: EL Stattin, P Henning, J Klar, E McDermott, C Stecksen-B, PE Sandström, TG Kellgren, P Rydén, G Hallmans, T Lönnerholm, A Ameur, MH Helfrich, FP Coxon, N Dahl, J Wikström, UH Lerner
    Sci Rep, 2017;7(1):3012.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Imprime PGG-Mediated Anti-Cancer Immune Activation Requires Immune Complex Formation
    PLoS ONE, 2016;11(11):e0165909.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Glioma-induced inhibition of caspase-3 in microglia promotes a tumor-supportive phenotype
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Curcumin inhibits osteoclastogenic potential in PBMCs from rheumatoid arthritis patients via the suppression of MAPK/RANK/c-Fos/NFATc1 signaling pathways
    Mol Med Rep, 2016;14(4):3620-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. oxLDL and eLDL Induced Membrane Microdomains in Human Macrophages
    PLoS ONE, 2016;11(11):e0166798.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Heme Oxygenase-1 Regulates Inflammation and Mycobacterial Survival in Human Macrophages during Mycobacterium tuberculosis Infection
    J Immunol, 2016;196(11):4641-9.
    Species: Human
    Sample Type: Plasma
    Application: Bioassay
  18. MARCO variants are associated with phagocytosis, pulmonary tuberculosis susceptibility and Beijing lineage
    Genes Immun., 2016;17(7):419-425.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Identification of Nedd9 as a TGF-?-Smad2/3 Target Gene Involved in RANKL-Induced Osteoclastogenesis by Comprehensive Analysis
    Authors: Yasunori Omata
    PLoS ONE, 2016;11(6):e0157992.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. MicroRNAs: Potential Biomarkers and Therapeutic Targets for Alveolar Bone Loss in Periodontal Disease
    Int J Mol Sci, 2016;17(8):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Nkx2-5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E-Deficient Mice
    J Am Heart Assoc, 2016;5(12):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Plasmacytoid dendritic cells and myeloid cells differently contribute to BAFF over-expression during primary HIV infection.
    Authors: Borhis G, Burelout C, Chaoul N, Smith N, Goujard C, Meyer L, Paul S, Saoudin H, Hosmalin A, Gilbert C, Herbeuval J, Richard Y
    AIDS, 2016;30(0):365-376.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Separable roles for Mycobacterium tuberculosis ESX-3 effectors in iron acquisition and virulence.
    Authors: Tufariello J, Chapman J, Kerantzas C, Wong K, Vilcheze C, Jones C, Cole L, Tinaztepe E, Thompson V, Fenyo D, Niederweis M, Ueberheide B, Philips J, Jacobs W
    Proc Natl Acad Sci U S A, 2016;113(3):E348-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Serum YKL-40 as a marker of liver fibrosis in patients with non-alcoholic fatty liver disease
    Sci Rep, 2016;6(0):35282.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Granulocyte-macrophage colony stimulatory factor enhances the pro-inflammatory response of interferon-gamma-treated macrophages to Pseudomonas aeruginosa infection.
    Authors: Singh S, Barr H, Liu Y, Robins A, Heeb S, Williams P, Fogarty A, Camara M, Martinez-Pomares L
    PLoS ONE, 2015;10(2):e0117447.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Disulfiram attenuates osteoclast differentiation in vitro: a potential antiresorptive agent.
    Authors: Ying H, Qin A, Cheng T, Pavlos N, Rea S, Dai K, Zheng M
    PLoS ONE, 2015;10(4):e0125696.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Interferon-gamma Inhibits Ebola Virus Infection.
    Authors: Rhein B, Powers L, Rogers K, Anantpadma M, Singh B, Sakurai Y, Bair T, Miller-Hunt C, Sinn P, Davey R, Monick M, Maury W
    PLoS Pathog, 2015;11(11):e1005263.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015;195(6):2818-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation.
    Authors: Mediero A, Ramkhelawon B, Perez-Aso M, Moore K, Cronstein B
    J Bone Miner Res, 2015;30(5):837-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. Xanthine oxidoreductase regulates macrophage IL1beta secretion upon NLRP3 inflammasome activation.
    Authors: Ives A, Nomura J, Martinon F, Roger T, LeRoy D, Miner J, Simon G, Busso N, So A
    Nat Commun, 2015;6(0):6555.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Glycogen synthase kinase 3beta ubiquitination by TRAF6 regulates TLR3-mediated pro-inflammatory cytokine production.
    Authors: Ko R, Park J, Ha H, Choi Y, Lee S
    Nat Commun, 2015;6(0):6765.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  32. The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis.
    Authors: Kim H, Kim K, Kim B, Cho M, Lee S
    PLoS ONE, 2015;10(4):e0124909.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  33. Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cells.
    Authors: Purushothaman P, Thakker S, Verma S
    J Virol, 2015;89(6):3093-111.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis.
    Authors: Lachmandas E, Vrieling F, Wilson L, Joosten S, Netea M, Ottenhoff T, van Crevel R
    PLoS ONE, 2015;10(2):e0117941.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. Impaired bone homeostasis in amyotrophic lateral sclerosis mice with muscle atrophy.
    Authors: Zhu K, Yi J, Xiao Y, Lai Y, Song P, Zheng W, Jiao H, Fan J, Wu C, Chen D, Zhou J, Xiao G
    J Biol Chem, 2015;290(13):8081-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  36. TLR8 Senses Bacterial RNA in Human Monocytes and Plays a Nonredundant Role for Recognition of Streptococcus pyogenes.
    Authors: Eigenbrod T, Pelka K, Latz E, Kreikemeyer B, Dalpke A
    J Immunol, 2015;195(3):1092-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  37. Genomewide Comprehensive Analysis Reveals Critical Cooperation Between Smad and c-Fos in RANKL-Induced Osteoclastogenesis.
    Authors: Omata Y, Yasui T, Hirose J, Izawa N, Imai Y, Matsumoto T, Masuda H, Tokuyama N, Nakamura S, Tsutsumi S, Yasuda H, Okamoto K, Takayanagi H, Hikita A, Imamura T, Matsuo K, Saito T, Kadono Y, Aburatani H, Tanaka S
    J Bone Miner Res, 2015;30(5):869-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  38. Human osteoclasts are inducible immunosuppressive cells in response to T cell-derived IFN-gamma and CD40 ligand in vitro.
    Authors: Li H, Lu Y, Qian J, Zheng Y, Zhang M, Bi E, He J, Liu Z, Xu J, Gao J, Yi Q
    J Bone Miner Res, 2014;29(12):2666-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  39. Laser ablation-inductively coupled plasma mass spectrometry: an emerging technology for detecting rare cells in tissue sections.
    Authors: Managh A, Hutchinson R, Riquelme P, Broichhausen C, Wege A, Ritter U, Ahrens N, Koehl G, Walter L, Florian C, Schlitt H, Reid H, Geissler E, Sharp B, Hutchinson J
    J Immunol, 2014;193(5):2600-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  40. TREM-1 is induced in tumor associated macrophages by cyclo-oxygenase pathway in human non-small cell lung cancer.
    Authors: Yuan, Zhihong, Mehta, Hiren J, Mohammed, Kamal, Nasreen, Najmunis, Roman, Robert, Brantly, Mark, Sadikot, Ruxana T
    PLoS ONE, 2014;9(5):e94241.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  41. miR-27a regulates inflammatory response of macrophages by targeting IL-10.
    Authors: Xie N, Cui H, Banerjee S, Tan Z, Salomao R, Fu M, Abraham E, Thannickal V, Liu G
    J Immunol, 2014;193(1):327-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  42. A TNFSF15 disease-risk polymorphism increases pattern-recognition receptor-induced signaling through caspase-8-induced IL-1.
    Authors: Hedl M, Abraham C
    Proc Natl Acad Sci U S A, 2014;111(37):13451-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  43. Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
    Authors: Yuan Z, Syed M, Panchal D, Joo M, Colonna M, Brantly M, Sadikot R
    J Biol Chem, 2014;289(21):15118-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  44. STAT1 interaction with E3-14.7K in monocytes affects the efficacy of oncolytic adenovirus.
    Authors: Spurrell, Emma, Gangeswaran, Rathi, Wang, Pengju, Cao, Fengyu, Gao, Dongling, Feng, Baisui, Wold, William, Tollefson, Ann, Lemoine, Nicholas, Wang, Yaohe
    J Virol, 2014;88(4):2291-300.
  45. MafB promotes atherosclerosis by inhibiting foam-cell apoptosis.
    Authors: Hamada, Michito, Nakamura, Megumi, Tran, Mai Thi, Moriguchi, Takashi, Hong, Cynthia, Ohsumi, Takayuki, Dinh, Tra Thi, Kusakabe, Manabu, Hattori, Motochik, Katsumata, Tokio, Arai, Satoko, Nakashima, Katsuhik, Kudo, Takashi, Kuroda, Etsushi, Wu, Chien-Hu, Kao, Pei-Han, Sakai, Masaharu, Shimano, Hitoshi, Miyazaki, Toru, Tontonoz, Peter, Takahashi, Satoru
    Nat Commun, 2014;5(0):3147.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  46. Macrophages inhibit human osteosarcoma cell growth after activation with the bacterial cell wall derivative liposomal muramyl tripeptide in combination with interferon-gamma.
    Authors: Pahl J, Kwappenberg K, Varypataki E, Santos S, Kuijjer M, Mohamed S, Wijnen J, van Tol M, Cleton-Jansen A, Egeler R, Jiskoot W, Lankester A, Schilham M
    J Exp Clin Cancer Res, 2014;33(0):27.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  47. Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.
    Authors: Giese, Sebastia, Marsh, Mark
    PLoS Pathog, 2014;10(7):e1004189.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  48. Phenotypic dissection of bone mineral density reveals skeletal site specificity and facilitates the identification of novel loci in the genetic regulation of bone mass attainment.
    Authors: Kemp J, Medina-Gomez C, Estrada K, St Pourcain B, Heppe D, Warrington N, Oei L, Ring S, Kruithof C, Timpson N, Wolber L, Reppe S, Gautvik K, Grundberg E, Ge B, van der Eerden B, van de Peppel J, Hibbs M, Ackert-Bicknell C, Choi K, Koller D, Econs M, Williams F, Foroud T, Zillikens M, Ohlsson C, Hofman A, Uitterlinden A, Davey Smith G, Jaddoe V, Tobias J, Rivadeneira F, Evans D
    PLoS Genet, 2014;10(6):e1004423.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  49. A GPBAR1 (TGR5) small molecule agonist shows specific inhibitory effects on myeloid cell activation in vitro and reduces experimental autoimmune encephalitis (EAE) in vivo.
    Authors: Lewis, Nuruddee, Patnaude, Lori A, Pelletier, Josephin, Souza, Donald J, Lukas, Susan M, King, F James, Hill, Jonathan, Stefanopoulos, Dimitria, Ryan, Kelli, Desai, Sudha, Skow, Donna, Kauschke, Stefan G, Broermann, Andre, Kuzmich, Daniel, Harcken, Christia, Hickey, Eugene R, Modis, Louise K
    PLoS ONE, 2014;9(6):e100883.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  50. Comparative analysis of the capacity of elite suppressor CD4+ and CD8+ T cells to inhibit HIV-1 replication in monocyte-derived macrophages.
    Authors: Walker-Sperling V, Buckheit R, Blankson J
    J Virol, 2014;88(17):9789-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  51. Tie2 signaling cooperates with TNF to promote the pro-inflammatory activation of human macrophages independently of macrophage functional phenotype.
    Authors: Garcia S, Krausz S, Ambarus C, Fernandez B, Hartkamp L, van Es I, Hamann J, Baeten D, Tak P, Reedquist K
    PLoS ONE, 2014;9(1):e82088.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  52. In vivo detection of macrophage recruitment in hind-limb ischemia using a targeted near-infrared fluorophore.
    Authors: Yoo, Jung Sun, Das, Raj Kuma, Jow, Zhi Yen, Chang, Young-Ta
    PLoS ONE, 2014;9(7):e103721.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  53. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase.
    Authors: Tang, Songqing, Chen, Taoyong, Yu, Zhou, Zhu, Xuhui, Yang, Mingjin, Xie, Bin, Li, Nan, Cao, Xuetao, Wang, Jianli
    Nat Commun, 2014;5(0):4657.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  54. COPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils.
    Authors: Eltboli O, Bafadhel M, Hollins F, Wright A, Hargadon B, Kulkarni N, Brightling C
    BMC Pulm Med, 2014;14(0):112.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  55. Growth factor and Th2 cytokine signaling pathways converge at STAT6 to promote arginase expression in progressive experimental visceral leishmaniasis.
    Authors: Osorio E, Travi B, da Cruz A, Saldarriaga O, Medina A, Melby P
    PLoS Pathog, 2014;10(6):e1004165.
  56. MiR-503 regulates osteoclastogenesis via targeting RANK.
    Authors: Chen C, Cheng P, Xie H, Zhou H, Wu X, Liao E, Luo X
    J Bone Miner Res, 2014;29(2):338-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  57. Monocyte to macrophage differentiation goes along with modulation of the plasmalogen pattern through transcriptional regulation.
    Authors: Wallner S, Grandl M, Konovalova T, Sigruner A, Kopf T, Peer M, Orso E, Liebisch G, Schmitz G
    PLoS ONE, 2014;9(4):e94102.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  58. NRROS negatively regulates reactive oxygen species during host defence and autoimmunity.
    Authors: Noubade, Rajkumar, Wong, Kit, Ota, Naruhisa, Rutz, Sascha, Eidenschenk, Celine, Valdez, Patricia, Ding, Jiabing, Peng, Ivan, Sebrell, Andrew, Caplazi, Patrick, DeVoss, Jason, Soriano, Robert H, Sai, Tao, Lu, Rongze, Modrusan, Zora, Hackney, Jason, Ouyang, Wenjun
    Nature, 2014;509(7499):235-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  59. The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways.
    Authors: Hedl M, Zheng S, Abraham C
    J Immunol, 2014;192(12):5924-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  60. Tumor necrosis factor stimulates osteoclastogenesis from human bone marrow cells under hypoxic conditions.
    Authors: Nomura T, Aoyama M, Waguri-Nagaya Y, Goto Y, Suzuki M, Miyazawa K, Asai K, Goto S
    Exp Cell Res, 2014;321(2):167-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  61. Ocaratuzumab, an Fc-engineered antibody demonstrates enhanced antibody-dependent cell-mediated cytotoxicity in chronic lymphocytic leukemia.
    Authors: Cheney C, Stephens D, Mo X, Rafiq S, Butchar J, Flynn J, Jones J, Maddocks K, O'Reilly A, Ramachandran A, Tridandapani S, Muthusamy N, Byrd J
    MAbs, 2014;6(3):749-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  62. GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer.
    Authors: Nebiker C, Han J, Eppenberger-Castori S, Iezzi G, Hirt C, Amicarella F, Cremonesi E, Huber X, Padovan E, Angrisani B, Droeser R, Rosso R, Bolli M, Oertli D, von Holzen U, Adamina M, Muraro M, Mengus C, Zajac P, Sconocchia G, Zuber M, Tornillo L, Terracciano L, Spagnoli G
    Clin Cancer Res, 2014;20(12):3094-106.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  63. Conditioned media from macrophages of M1, but not M2 phenotype, inhibit the proliferation of the colon cancer cell lines HT-29 and CACO-2.
    Authors: Engstrom A, Erlandsson A, Delbro D, Wijkander J
    Int J Oncol, 2014;44(2):385-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  64. Maresin biosynthesis and identification of maresin 2, a new anti-inflammatory and pro-resolving mediator from human macrophages.
    Authors: Deng B, Wang C, Arnardottir H, Li Y, Cheng C, Dalli J, Serhan C
    PLoS ONE, 2014;9(7):e102362.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  65. The CD37-targeted antibody-drug conjugate IMGN529 is highly active against human CLL and in a novel CD37 transgenic murine leukemia model.
    Authors: Beckwith K, Frissora F, Stefanovski M, Towns W, Cheney C, Mo X, Deckert J, Croce C, Flynn J, Andritsos L, Jones J, Maddocks K, Lozanski G, Byrd J, Muthusamy N
    Leukemia, 2014;28(7):1501-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  66. Differential responses of disease-resistant and disease-susceptible primate macrophages and myeloid dendritic cells to simian hemorrhagic fever virus infection.
    Authors: Vatter H, Brinton M
    J Virol, 2014;88(4):2095-106.
    Species: Primate - Baboon
    Sample Type: Whole Cells
    Application: Bioassay
  67. The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4.
    Authors: van Wilgenburg, Bonnie, Moore, Michael, James, William, Cowley, Sally A
    PLoS ONE, 2014;9(1):e86071.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  68. Epstein-Barr virus infection induces indoleamine 2,3-dioxygenase expression in human monocyte-derived macrophages through p38/mitogen-activated protein kinase and NF-kappaB pathways: impairment in T cell functions.
    Authors: Liu W, Lin Y, Xiao H, Xing S, Chen H, Chi P, Zhang G
    J Virol, 2014;88(12):6660-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  69. Selective tyrosine kinase inhibition of insulin-like growth factor-1 receptor inhibits human and mouse breast cancer-induced bone cell activity, bone remodeling, and osteolysis.
    Authors: Logan J, Sophocleous A, Marino S, Muir M, Brunton V, Idris A
    J Bone Miner Res, 2013;28(5):1229-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  70. Functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele.
    Authors: Kauder, Steven E, Santell, Lydia, Mai, Elaine, Wright, Lilyan Y, Luis, Elizabet, N'Diaye, Elsa N, Lutman, Jeff, Ratti, Navneet, Sa, Susan M, Maun, Henry R, Stefanich, Eric, Gonzalez, Lino C, Graham, Robert R, Diehl, Lauri, Faubion, William, Keir, Mary E, Young, Judy, Chaudhuri, Amitabha, Lazarus, Robert A, Egen, Jackson
    PLoS ONE, 2013;8(12):e83958.
    Species: Human
    Sample Type: Whole Cells
    Application: cell culture
  71. Bile acids PKA-dependently induce a switch of the IL-10/IL-12 ratio and reduce proinflammatory capability of human macrophages.
    Authors: Haselow K, Bode J, Wammers M, Ehlting C, Keitel V, Kleinebrecht L, Schupp A, Haussinger D, Graf D
    J Leukoc Biol, 2013;94(6):1253-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  72. HSP90 inhibitors enhance differentiation and MITF (microphthalmia transcription factor) activity in osteoclast progenitors.
    Authors: van der Kraan A, Chai R, Singh P, Lang B, Xu J, Gillespie M, Price J, Quinn J
    Biochem J, 2013;451(2):235-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  73. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
    Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
    Blood, 2013;121(1):95-106.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  74. A human ICAM-1 antibody isolated by a function-first approach has potent macrophage-dependent antimyeloma activity in vivo.
    Authors: Veitonmaki N, Hansson M, Zhan F, Sundberg A, Lofstedt T, Ljungars A, Li Z, Martinsson-Niskanen T, Zeng M, Yang Y, Danielsson L, Kovacek M, Lundqvist A, Martensson L, Teige I, Tricot G, Frendeus B
    Cancer Cell, 2013;23(4):502-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  75. Early estrogen-induced gene 1, a novel RANK signaling component, is essential for osteoclastogenesis.
    Authors: Choi H, Kang H, Jung E, Kim T, Lin J, Lee S
    Cell Res, 2013;23(4):524-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  76. TGR5 signalling inhibits the production of pro-inflammatory cytokines by in vitro differentiated inflammatory and intestinal macrophages in Crohn's disease.
    Authors: Yoneno K, Hisamatsu T, Shimamura K, Kamada N, Ichikawa R, Kitazume M, Mori M, Uo M, Namikawa Y, Matsuoka K, Sato T, Koganei K, Sugita A, Kanai T, Hibi T
    Immunology, 2013;139(1):19-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  77. Adenosine regulates bone metabolism via A1, A2A, and A2B receptors in bone marrow cells from normal humans and patients with multiple myeloma.
    Authors: He W, Mazumder A, Wilder T, Cronstein B
    FASEB J, 2013;27(9):3446-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  78. Bone marrow stromal cells derived MCP-1 reverses the inhibitory effects of multiple myeloma cells on osteoclastogenesis by upregulating the RANK expression.
    Authors: Liu Z, Xu J, Li H, Zheng Y, He J, Liu H, Zhong Y, Lu Y, Hong B, Zhang M, Lin P, Du J, Hou J, Qian J, Kwak L, Yi Q, Yang J
    PLoS ONE, 2013;8(12):e82453.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  79. Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis.
    Authors: Johnston A, Xing X, Swindell W, Kochkodan J, Riblett M, Nair R, Stuart P, Ding J, Voorhees J, Elder J, Gudjonsson J
    Hum Mol Genet, 2013;22(9):1807-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  80. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  81. HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes.
    Authors: Lan X, Xu J, Kiyota T, Peng H, Zheng JC, Ikezu T
    J. Immunol., 2011;186(12):6925-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  82. Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection.
    Authors: Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W
    J. Immunol., 2011;187(2):748-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  83. A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.
    Authors: Mengshol JA, Golden-Mason L, Arikawa T
    PLoS ONE, 2010;5(3):e9504.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  84. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010;28(5):939-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  85. Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4.
    Authors: Knowles HJ, Cleton-Jansen AM, Korsching E, Athanasou NA
    FASEB J., 2010;24(12):4648-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  86. Characterisation of the osteoclastogenic potential of human osteoblastic and fibroblastic conditioned media.
    Authors: Costa-Rodrigues J, Teixeira CA, Sampaio P, Fernandes MH
    J. Cell. Biochem., 2010;109(1):205-16.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  87. Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma.
    Authors: Lamprecht B, Walter K, Kreher S, Kumar R, Hummel M, Lenze D, Kochert K, Bouhlel MA, Richter J, Soler E, Stadhouders R, Johrens K, Wurster KD, Callen DF, Harte MF, Giefing M, Barlow R, Stein H, Anagnostopoulos I, Janz M, Cockerill PN, Siebert R, Dorken B, Bonifer C, Mathas S
    Nat. Med., 2010;16(5):571-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  88. Glucocorticoids maintain human osteoclasts in the active mode of their resorption cycle.
    Authors: Soe K, Delaisse JM
    J. Bone Miner. Res., 2010;25(10):2184-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  89. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
  90. IL-3 inhibits human osteoclastogenesis and bone resorption through downregulation of c-Fms and diverts the cells to dendritic cell lineage.
    Authors: Gupta N, Barhanpurkar AP, Tomar GB
    J. Immunol., 2010;185(4):2261-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  91. Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
    Authors: Mabilleau G, Sabokbar A
    PLoS ONE, 2009;4(1):e4173.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  92. Prostate cancer promotes CD11b positive cells to differentiate into osteoclasts.
    Authors: Mizutani K, Sud S, Pienta KJ
    J. Cell. Biochem., 2009;106(4):563-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  93. The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.
    Authors: Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P
    Biomaterials, 2009;30(20):3415-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  94. Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts.
    Authors: Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C
    Bone, 2009;44(6):1143-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Media Supplement - Cell Prep
  95. Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients.
    Authors: Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M
    Leukemia, 2009;23(11):2139-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  96. Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesis.
    Authors: Chen H, Campbell RA, Chang Y, Li M, Wang CS, Li J, Sanchez E, Share M, Steinberg J, Berenson A, Shalitin D, Zeng Z, Gui D, Perez-Pinera P, Berenson RJ, Said J, Bonavida B, Deuel TF, Berenson JR
    Blood, 2009;113(9):1992-2002.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  97. Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis.
    Authors: Irie N, Takada Y, Watanabe Y, Matsuzaki Y, Naruse C, Asano M, Iwakura Y, Suda T, Matsuo K
    J. Biol. Chem., 2009;284(21):14637-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  98. Maturing dendritic cells are an important source of IL-29 and IL-20 that may cooperatively increase the innate immunity of keratinocytes.
    Authors: Wolk K, Witte K, Witte E, Proesch S, Schulze-Tanzil G, Nasilowska K, Thilo J, Asadullah K, Sterry W, Volk HD, Sabat R
    J. Leukoc. Biol., 2008;83(5):1181-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  99. Activation of the receptor EphB4 by its specific ligand ephrin B2 in human osteoarthritic subchondral bone osteoblasts.
    Authors: Kwan Tat S, Pelletier JP, Amiable N, Boileau C, Lajeunesse D, Duval N, Martel-Pelletier J
    Arthritis Rheum., 2008;58(12):3820-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  100. p38 mitogen-activated protein kinase inhibitor LY2228820 enhances bortezomib-induced cytotoxicity and inhibits osteoclastogenesis in multiple myeloma; therapeutic implications.
    Authors: Ishitsuka K, Hideshima T, Neri P, Vallet S, Shiraishi N, Okawa Y, Shen Z, Raje N, Kiziltepe T, Ocio EM, Chauhan D, Tassone P, Munshi N, Campbell RM, Dios AD, Shih C, Starling JJ, Tamura K, Anderson KC
    Br. J. Haematol., 2008;141(5):598-606.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  101. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  102. Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
    Authors: Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD
    Atherosclerosis, 2008;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  103. Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway.
    Authors: Chamoux E, Houde N, L'Eriger K, Roux S
    J. Cell. Physiol., 2008;216(2):536-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  104. Lenalidomide inhibits osteoclastogenesis, survival factors and bone-remodeling markers in multiple myeloma.
    Authors: Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Mitsiades C, Chauhan D, Okawa Y, Munshi NC, Richardson PG, Anderson KC
    Leukemia, 2008;22(10):1925-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  105. Human anti-inflammatory macrophages induce Foxp3+ GITR+ CD25+ regulatory T cells, which suppress via membrane-bound TGFbeta-1.
    Authors: Savage ND, De Boer T, Walburg KV, Joosten SA, van Meijgaarden K, Geluk A, Ottenhoff TH
    J. Immunol., 2008;181(3):2220-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  106. Pulse treatment with the proteasome inhibitor bortezomib inhibits osteoclast resorptive activity in clinically relevant conditions.
    Authors: Boissy P, Andersen TL, Lund T, Kupisiewicz K, Plesner T, Delaisse JM
    Leuk. Res., 2008;32(11):1661-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  107. Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis.
    Authors: Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M
    J. Pathol., 2007;212(1):47-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  108. Targeting MEK1/2 blocks osteoclast differentiation, function and cytokine secretion in multiple myeloma.
    Authors: Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Chauhan D, Munshi NC, Richardson PG, Anderson KC
    Br. J. Haematol., 2007;139(1):55-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  109. Attenuation of bone mass and increase of osteoclast formation in decoy receptor 3 transgenic mice.
    Authors: Tang CH, Hsu TL, Lin WW, Lai MZ, Yang RS, Hsieh SL, Fu WM
    J. Biol. Chem., 2007;282(4):2346-54.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  110. Gingival fibroblasts are better at inhibiting osteoclast formation than periodontal ligament fibroblasts.
    Authors: de Vries TJ, Schoenmaker T, Wattanaroonwong N, van den Hoonaard M, Nieuwenhuijse A, Beertsen W, Everts V
    J. Cell. Biochem., 2006;98(2):370-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  111. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
    Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
    Arthritis Rheum., 2006;54(5):1451-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  112. Death receptors, Fas and TRAIL receptors, are involved in human osteoclast apoptosis.
    Authors: Roux S, Lambert-Comeau P, Saint-Pierre C, Lepine M, Sawan B, Parent JL
    Biochem. Biophys. Res. Commun., 2005;333(1):42-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  113. IL-1 mediates TNF-induced osteoclastogenesis.
    Authors: Wei S, Kitaura H, Zhou P, Ross FP, Teitelbaum SL
    J. Clin. Invest., 2005;115(2):282-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  114. Phenotype and function of myeloid dendritic cells derived from African green monkey blood monocytes.
    Authors: Mortara L, Ploquin MJ, Faye A, Scott-Algara D, Vaslin B, Butor C, Hosmalin A, Barre-Sinoussi F, Diop OM, Muller-Trutwin MC
    J. Immunol. Methods, 2005;308(1):138-55.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Whole Cells
    Application: Bioassay
  115. A disintegrin and metalloproteinase 10-mediated cleavage and shedding regulates the cell surface expression of CXC chemokine ligand 16.
    Authors: Gough PJ, Garton KJ, Wille PT, Rychlewski M, Dempsey PJ, Raines EW
    J. Immunol., 2004;172(6):3678-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  116. IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
    Authors: Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB
    J. Immunol., 2004;172(10):6476-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  117. Reprogramming of IL-10 activity and signaling by IFN-gamma.
    Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
    J. Immunol., 2003;171(10):5034-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 117 Citations
Animal-Free Recombinant Proteins
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Recombinant Human M-CSF, Animal-Free Protein

BA AFL216
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Average Rating: 4.7 (Based on 6 reviews)

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We have 5 reviews tested in 5 applications: Cell Differentiation, Stem/Immune cell maintenance or differentiation , Cell proliferation, Differentiating primary human monocytes to macrophages in vitro and use the macrophages for further functional assays, In vitro bioactivity in cell culture.
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Cell Differentiation Recombinant Human M-CSF Protein 216-MC
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 Cell Differentiation Anonymous 11/18/2017
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Cell Differentiation Recombinant Human M-CSF Protein 216-MC
Cell Differentiation: Recombinant Human M-CSF Protein [216-MC]

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ApplicationCell Differentiation

Other Experimental Details

Other Experimental DetailsMonocyte to Macrophage differentiation
  Very Good
  Anonymous 05/03/2017
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 Stem/Immune cell maintenance or differentiation Anonymous 12/15/2016
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Summary

ApplicationStem/Immune cell maintenance or differentiation

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Other Experimental DetailsWe use for blood cell differentiation.
Cell proliferation Recombinant Human M-CSF Protein 216-MC
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 Cell proliferation Anonymous 05/18/2016
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Cell proliferation Recombinant Human M-CSF Protein 216-MC
Cell proliferation: Recombinant Human M-CSF Protein [216-MC]

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ApplicationCell proliferation
  Excellent
 Differentiating primary human monocytes to macrophages in vitro and use the macrophages for further functional assays Anonymous 05/12/2016
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Summary

ApplicationDifferentiating primary human monocytes to macrophages in vitro and use the macrophages for further functional assays
Reason for RatingWorks very well for differentiation of primary human monocytes to macrophages.
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 In vitro bioactivity in cell culture Anonymous 01/21/2016
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ApplicationIn vitro bioactivity in cell culture

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