Human CD69 Fluorescein-conjugated Antibody

Name: Human CD69 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB23591F

Catalog #: FAB23591F Datasheet / COA / SDS

Newer Version Available: FAB23591G

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Discontinued Product

FAB23591F has been discontinued and is replaced by FAB23591G.

Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD69 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 298614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes untreated (open histogram, blue line) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB23591F). Treated lymphocytes were also stained with isotype control antibody (Catalog # IC003F, open histogram, orange line). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Alternate Names

activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60