Human CD69 Alexa Fluor® 488-conjugated Antibody

FAB23591G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB23591G-100UG

Bulk Orders

Human CD69 Alexa Fluor® 488-conjugated Antibody in Flow Cytometry

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD69 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 298614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

View Larger

Detection of CD69 in Activated Human PBMC by Flow Cytometry. Human PBMC (A) activated with anti-CD3 and anti-CD28 antibodies for 48 hours, or (B) untreated, were stained with Mouse Anti-Human CD69 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB23591G). Quadrant markers were set based on isotype control antibody staining (Catalog # IC003G). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry Human peripheral blood lymphocytes either untreated (open histogram) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB23591G) or isotype control antibody (Catalog # IC003G, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Alternate Names

activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60