Human CD69 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB23591G-100UG
Human CD69 Alexa Fluor® 488-conjugated Antibody in Flow Cytometry
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Human CD69 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.
Source
Monoclonal Mouse IgG2A Clone # 298614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD69 in Activated Human PBMC by Flow Cytometry. View Larger

Detection of CD69 in Activated Human PBMC by Flow Cytometry. Human PBMC (A) activated with anti-CD3 and anti-CD28 antibodies for 48 hours, or (B) untreated, were stained with Mouse Anti-Human CD69 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB23591G). Quadrant markers were set based on isotype control antibody staining (Catalog # IC003G). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry View Larger

Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry Human peripheral blood lymphocytes either untreated (open histogram) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB23591G) or isotype control antibody (Catalog # IC003G, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution


Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Entrez Gene IDs
969 (Human); 12515 (Mouse)
Alternate Names
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60

Product Datasheets

FAQs

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