Human CD69 Alexa Fluor® 488-conjugated Antibody

FAB23591G Datasheet / COA / SDS

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FAB23591G-100UG

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Human CD69 Alexa Fluor® 488-conjugated Antibody in Data

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Human CD69 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 298614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

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Detection of CD69 in Activated Human PBMC by Flow Cytometry. Human PBMC (A) activated with anti-CD3 and anti-CD28 antibodies for 48 hours, or (B) untreated, were stained with Mouse Anti-Human CD69 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB23591G). Quadrant markers were set based on isotype control antibody staining (Catalog # IC003G). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry Human peripheral blood lymphocytes either untreated (open histogram) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB23591G) or isotype control antibody (Catalog # IC003G, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Alternate Names

activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60