Human CD8 Antibody

Name: Human CD8 Antibody
Brand: R&D Systems
SKU: MAB11531
Price: 419 USD
Availability: InStock

R&D Systems | Catalog # MAB11531

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human

Applications

Cell depletion

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # 30798-1

View all CD8 Primary Antibodies »

Product Specifications

Immunogen

Alpha chain of CD8
Accession # P01732

Specificity

Detects recombinant human CD8 in direct ELISA

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Scientific Data Images for Human CD8 Antibody

Immobilized recombinant CD8 antibody supports the adhesion of SUP-T1 cells to cell culture plates.

The CD8 antibody was coated onto cell culture plates at increasing amounts. The wells were then incubated with calcein labeled SUP-T1 cells, and cell binding was detected using a fluorescence-based plate reader. The ED₅₀ for this effect is 0.200‑2.00 μg/mL.

Applications for Human CD8 Antibody

Application

Recommended Usage

Cell depletion

2 µg/mL
Sample: Selects CD8+ cells when immobilized to cell culture plate or bead

Formulation, Preparation, and Storage

Purification

Protein G purified from cell culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
*Small pack size (-SP) is shipped at ambient temperature or with polar packs. Upon receipt, store it immediately at the temperature

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD8

This Bio-Techne CD8 antibody is a reformatted version of the RPA-T8 clone. CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. There are two isoforms of the protein, alpha and beta, each encoded by a different gene. In humans, both genes are located on chromosome 2 in position 2p12. The most common form of CD8 is composed of a CD8-alpha and CD8-beta chain, both members of the immunoglobulin superfamily with an immunoglobulin variable (IgV)-like extracellular domain connected to the membrane by a thin stalk, and an intracellular tail. Less-common homodimers of the CD8-alpha chain are also expressed on some cells.