Human CD81 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4615R-100UG

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Detection of CD81 in Human Lymphocytes by Flow Cytometry.
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Product Details
Citations (1)
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Reviews (2)

Human CD81 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD81. Stains human CD81 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 454720
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human CD81
Accession # P60033
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD81 in Human Lymphocytes by Flow Cytometry. View Larger

Detection of CD81 in Human Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human CD81 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB4615R, filled histogram) or isotype control antibody (IC0041R, open histogram). Staining was performed using our Staining Membrane-Associated Proteins protocol.

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Preparation and Storage

Reconstitution
Reconstitution default not known
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD81

CD81, also known as TAPA-1 and TM4SF4, is a widely expressed protein in the tetraspanin family. CD81 is a multifunctional protein that interacts with a variety of other molecules, including tetraspanins, and is important for organization of the plasma membrane into microdomains. CD81 facilitates B cell and T cell activation and is an integrin-binding adhesion molecule. CD81 expression on lymphocytes is altered during infection by hepatitis C virus or HIV-1 and contributes to the pathogenicity of those viruses. Human CD81 shares 92%‑93% aa sequence identity with mouse and rat CD81.

Entrez Gene IDs
975 (Human); 12520 (Mouse); 25621 (Rat)
Alternate Names
CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human CD81 Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Synergy of Human Platelet-Derived Extracellular Vesicles with Secretome Proteins Promotes Regenerative Functions
    Authors: FG Gomes, AC Andrade, M Wolf, S Hochmann, L Krisch, N Maeding, C Regl, R Poupardin, P Ebner-Peki, CG Huber, N Meisner-Ko, K Schallmose, D Strunk
    Biomedicines, 2022;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human CD81 Alexa Fluor® 647-conjugated Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human CD81 Alexa Fluor® 647-conjugated Antibody
By Anonymous on 09/26/2022
Application: Flow Sample Tested: microglia Species: Human

Human CD81 Alexa Fluor® 647-conjugated Antibody
By Carlos Jesus on 05/10/2021
Application: Flow Sample Tested: Extracellular Vesicles Species: Human

Works better with fresh samples