Human CDC42 Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # FAB11765AFP405

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3193C
View all CDC42 Primary Antibodies »

Product Specifications

Specificity

Detects a synthetic peptide specific for human CDC32 around amino acid 140 in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human CDC42 Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CDC42

Cell division control protein 42 (CDC42) is a small Rho GTPase with a molecular weight of approximately 21 kDa. CDC42 is a highly conserved protein that plays an integral role in regulating multiple cellular processes, including cytoskeletal organization, cell polarity, vesicle trafficking, and signal transduction. A key regulator of the actin cytoskeleton, CDC42 is critical for the formation of filopodia, which are dynamic, actin-rich cell protrusions involved in sensing the extracellular environment. CDC42 is ubiquitously expressed across eukaryotic cells and contributes to essential biological functions such as cell migration, adhesion, and mitotic spindle orientation. Dysregulation of CDC42 activity is implicated in a variety of pathological conditions, including cancer metastasis, where it drives tumor cell migration and invasion, as well as immune disorders resulting from aberrant cell polarization and trafficking. Mutations in genes regulating CDC42 and its activity are associated with developmental disorders, such as Takenouchi-Kosaki syndrome. Given its central role in cellular signaling and cytoskeletal dynamics, CDC42 is a key player in numerous physiological and pathological contexts, making it a valuable biomarker and therapeutic target.

References

  1. Ridley AJ. Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking. Trends Cell Biol. 2006 Oct;16(10):522-9. doi: 10.1016/j.tcb.2006.08.006. Epub 2006 Sep 1. PMID: 16949823.
  2. Johnson DI. Cdc42: An essential Rho-type GTPase controlling eukaryotic cell polarity. Microbiol Mol Biol Rev. 1999 Mar;63(1):54-105. doi: 10.1128/MMBR.63.1.54-105.1999. PMID: 10066831; PMCID: PMC98957.
  3. Jahid S, Ortega JA, Vuong LM, Acquistapace IM, Hachey SJ, Flesher JL, La Serra MA, Brindani N, La Sala G, Manigrasso J, Arencibia JM, Bertozzi SM, Summa M, Bertorelli R, Armirotti A, Jin R, Liu Z, Chen CF, Edwards R, Hughes CCW, De Vivo M, Ganesan AK. Structure-based design of CDC42 effector interaction inhibitors for the treatment of cancer. Cell Rep. 2022 Apr 5;39(1):110641. doi: 10.1016/j.celrep.2022.110641. Erratum in: Cell Rep. 2022 Apr 26;39(4):110760. doi: 10.1016/j.celrep.2022.110760. PMID: 35385746; PMCID: PMC9127750.

Long Name

Cell Division Cycle 42

Alternate Names

G25K

Entrez Gene IDs

998 (Human)

Gene Symbol

CDC42

UniProt

P60953 (Human)

Additional CDC42 Products

Product Documents for Human CDC42 Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CDC42 Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Cell Cycle

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