Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3193C
View all CDC42 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # P60953

Specificity

Detects a synthetic peptide specific for human CDC32 around amino acid 140 in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human CDC42 Antibody

Detection of Human CDC42 by Western Blot.

Western Blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human CDC42 Monoclonal Antibody (Catalog # MAB11765) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CDC42 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human CDC42 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDC42

Cell division control protein 42 (CDC42) is a small Rho GTPase with a molecular weight of approximately 21 kDa. CDC42 is a highly conserved protein that plays an integral role in regulating multiple cellular processes, including cytoskeletal organization, cell polarity, vesicle trafficking, and signal transduction. A key regulator of the actin cytoskeleton, CDC42 is critical for the formation of filopodia, which are dynamic, actin-rich cell protrusions involved in sensing the extracellular environment. CDC42 is ubiquitously expressed across eukaryotic cells and contributes to essential biological functions such as cell migration, adhesion, and mitotic spindle orientation. Dysregulation of CDC42 activity is implicated in a variety of pathological conditions, including cancer metastasis, where it drives tumor cell migration and invasion, as well as immune disorders resulting from aberrant cell polarization and trafficking. Mutations in genes regulating CDC42 and its activity are associated with developmental disorders, such as Takenouchi-Kosaki syndrome. Given its central role in cellular signaling and cytoskeletal dynamics, CDC42 is a key player in numerous physiological and pathological contexts, making it a valuable biomarker and therapeutic target.

References

  1. Ridley AJ. Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking. Trends Cell Biol. 2006 Oct;16(10):522-9. doi: 10.1016/j.tcb.2006.08.006. Epub 2006 Sep 1. PMID: 16949823.
  2. Johnson DI. Cdc42: An essential Rho-type GTPase controlling eukaryotic cell polarity. Microbiol Mol Biol Rev. 1999 Mar;63(1):54-105. doi: 10.1128/MMBR.63.1.54-105.1999. PMID: 10066831; PMCID: PMC98957.
  3. Jahid S, Ortega JA, Vuong LM, Acquistapace IM, Hachey SJ, Flesher JL, La Serra MA, Brindani N, La Sala G, Manigrasso J, Arencibia JM, Bertozzi SM, Summa M, Bertorelli R, Armirotti A, Jin R, Liu Z, Chen CF, Edwards R, Hughes CCW, De Vivo M, Ganesan AK. Structure-based design of CDC42 effector interaction inhibitors for the treatment of cancer. Cell Rep. 2022 Apr 5;39(1):110641. doi: 10.1016/j.celrep.2022.110641. Erratum in: Cell Rep. 2022 Apr 26;39(4):110760. doi: 10.1016/j.celrep.2022.110760. PMID: 35385746; PMCID: PMC9127750.

Long Name

Cell Division Cycle 42

Alternate Names

G25K

Entrez Gene IDs

998 (Human)

Gene Symbol

CDC42

UniProt

P60953 (Human)

Additional CDC42 Products

Product Documents for Human CDC42 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CDC42 Antibody

For research use only

Related Research Areas

Cell Cycle

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