CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 934660
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Product Specifications
Immunogen
E. coli-derived recombinant human CEACAM-7
Asn37-Phe142
Accession # Q14002
Asn37-Phe142
Accession # Q14002
Specificity
Detects human CEACAM-7 in direct ELISAs. Stains human CEACAM-7 transfected cells but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CEACAM‑7 Antibody
Detection of CEACAM‑7 in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP was stained with either (A) Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB4478) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Human CEACAM‑7 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CEACAM-7
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 7
Alternate Names
CEACAM7, CGM2
Entrez Gene IDs
1087 (Human)
Gene Symbol
CEACAM7
UniProt
Additional CEACAM-7 Products
Product Documents for Human CEACAM‑7 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CEACAM‑7 Antibody
For research use only
Related Research Areas
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