Human CEACAM-7 Antibody

Catalog # Availability Size / Price Qty
MAB4478
MAB4478-SP
Detection of CEACAM‑7 in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP by Flow Cytometry.
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Human CEACAM-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM-7 in direct ELISAs. Stains human CEACAM-7 transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 934660
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CEACAM-7
Asn37-Phe142
Accession # Q14002
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CEACAM‑7 in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP by Flow Cytometry. View Larger

Detection of CEACAM‑7 in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP was stained with either (A) Mouse Anti-Human CEACAM‑7 Monoclonal Antibody (Catalog # MAB4478) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-7

CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235.

Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 7
Entrez Gene IDs
1087 (Human)
Alternate Names
Carcinoembryonic antigen CGM 2; Carcinoembryonic antigen CGM2; Carcinoembryonic antigen gene family member 2; Carcinoembryonic antigen related cell adhesion molecule 7; CEA; CEACAM 7; CEACAM7; CEACAM-7; CGM 2; CGM2

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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