Human CEACAM-7 Antibody

Catalog # Availability Size / Price Qty
MAB44781-100
MAB44781-SP

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Detection of CEACAM‑7 in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP by Flow Cytometry.
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Human CEACAM-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM-7 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 962703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived recombinant human CEACAM-7
Thr36-Asn233
Accession # Q14002
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human CEACAM‑7 Monoclonal Antibody (Catalog # MAB44782).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CEACAM-7 DuoSet ELISA Kit (Catalog # DY4478-05) for convenient development of a sandwich ELISA.

 
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CEACAM-7 antibody in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP antibody by Flow Cytometry. View Larger

Detection of CEACAM‑7 in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP was stained with either (A) Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB44781) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry CEACAM-7 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

CEACAM‑7 in Human Colon Cancer Tissue. CEACAM-7 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB44781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human CEACAM‑7 ELISA Standard Curve. Recombinant Human CEACAM-7 protein was serially diluted 2-fold and captured by Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB44782) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB44781) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-7

Carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM-7), also known as CGM2, is an approximately 40 kDa GPI-anchored glycoprotein in the CEACAM family of adhesion molecules (1). Mature human CEACAM-7 consists of two Ig-like domains followed by the GPI anchor (2). Alternative splicing generates a short isoform that lacks the second Ig-like domain. CEACAM-7 is preferentially expressed on the luminal surface of epithelial cells near the mouth of colonic crypts and on pancreatic ductal epithelial cells (3, 4). It is down-regulated during colorectal adenoma progression (2-6) but can be up-regulated during the development of gastric carcinoma (7). R&D Systems in-house testing indicates that CEACAM-7 binds to CEACAM-1, consistent with the heterophilic interaction of CEACAM-1 with other CEACAM family members (1, 8, 9).

References
  1. Tchoupa, A.K. et al. (2014) Cell Commun. Signal. 12:27.
  2. Thompson, J. et al. (1994) J. Biol. Chem. 269:32924.
  3. Thompson, J. et al. (1997) Cancer Res. 57:1776.
  4. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
  5. Nollau, P. et al. (1997) Cancer Res. 57:2354.
  6. Nollau, P. et al. (1997) Am. J. Pathol. 151:521.
  7. Zhou, J. et al. (2011) World J. Surgical Oncol. 9:172.
  8. Markel, G. et al. (2004) J. Immunol. 173:3732.
  9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 7
Entrez Gene IDs
1087 (Human)
Alternate Names
Carcinoembryonic antigen CGM 2; Carcinoembryonic antigen CGM2; Carcinoembryonic antigen gene family member 2; Carcinoembryonic antigen related cell adhesion molecule 7; CEA; CEACAM 7; CEACAM7; CEACAM-7; CGM 2; CGM2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CEACAM-7 Antibody

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Human CEACAM-7 Antibody
By Anonymous on 11/30/2021
Application: Immunohistochemistry Sample Tested: Gastric carcinoma Species: Human