Human Chemerin Biotinylated Antibody

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Product Details
Citations (4)
Supplemental Products

Human Chemerin Biotinylated Antibody Summary

Species Reactivity
Detects human Chemerin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse Chemerin is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human Chemerin (R&D Systems, Catalog # 2324-CM)
Accession # Q99969
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Chemerin (Catalog # 2324-CM)

Human Chemerin Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Chemerin Antibody (Catalog # MAB23241)

Standard: Recombinant Human Chemerin Protein (Catalog # 2324-CM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chemerin

Human Chemerin, also known as Tazarotene-induced Gene 2, (TIG2) is a new, but distant member of the Cystatin superfamily (1‑3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (2, 3). Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin-fold containing domain, and a six aa C-terminal prosegment (1, 4). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the fold, and three potential sites for phosphorylation and one for myristoylation (5). The precursor molecule undergoes proteolytic processing at both termini by unknown proteases. The N-terminal residue 20 aa hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (1, 6). In either case, it gives rise to a soluble proform that undergoes further processing at the C-terminus. In human, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 21‑157) (7). A shorter 134 aa form has been described (5). Bioactivity seems to be concentrated in the nine residues preceding the prosegment (aa 149‑157). Retention of the prosegment blocks activity (4). The 137 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (7). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature human Chemerin shares 67% aa sequence identity with mouse Chemerin (7). There is apparently cross-species activity for the protein (8).

  1. Nagpal, S. et al., (1997) J. Invest. Dermatol. 109:91.
  2. Storici, P. et al., (1996) Eur. J. Biochem. 238:769.
  3. Zanetti, M., (2004) J. Leukoc. Biol. 75:39.
  4. Wittamer, V. et al., (2004) J. Biol. Chem. 279:9956.
  5. Meder, W. et al., (2003) FEBS Lett. 555:495.
  6. Yokoyama-Kobayashi, M. et al., (1999) Gene 228:161.
  7. Wittamer, V. et al., (2003) J. Exp. Med. 198:977.
  8. Busmann, A. et al., (2004) J. Chromatog. B 811:217.
Long Name
Retinoic Acid Receptor Responder Protein 2
Entrez Gene IDs
5919 (Human); 71660 (Mouse)
Alternate Names
Chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433

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Product Specific Notices

This product and/or its use is the subject of European Patent 1 405 083 B1, US Patents 7,332,291; 7,419,658 and 7,842,453 as well as foreign equivalents licensed to R&D Systems Inc. The purchase of this product is intended for research purposes only, not including the screening of compounds for the development of therapeutic and/or diagnostic products. Buyers may require a separate license to the patent rights for applications beyond such research purposes. For information on licensing please contact Euroscreen SA rue Adrienne Bolland n°47 B-6041 Gosselies Belgium. Phone: +32-71-348500, Fax: +32-71-348519, e-mail Attention: Dr. Vincent Lannoy.

Citations for Human Chemerin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Antimicrobial and Attractant Roles for Chemerin in the Oral Cavity during Inflammatory Gum Disease
    Authors: U Godlewska, P Brzoza, A Sroka, P Majewski, H Jentsch, M Eckert, S Eick, J Potempa, BA Zabel, J Cichy
    Front Immunol, 2017;8(0):353.
    Species: Human
    Sample Types: Serum
  2. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  3. Potential role of chemerin in recruitment of plasmacytoid dendritic cells to diseased skin.
    Authors: Skrzeczynska-Moncznik J, Wawro K, Stefanska A, Oleszycka E, Kulig P, Zabel BA, Sulkowski M, Kapinska-Mrowiecka M, Czubak-Macugowska M, Butcher EC, Cichy J
    Biochem. Biophys. Res. Commun., 2009;380(2):323-7.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Regulation of chemerin bioactivity by plasma carboxypeptidase N, carboxypeptidase B (activated thrombin-activable fibrinolysis inhibitor), and platelets.
    Authors: Du XY, Zabel BA, Myles T, Allen SJ, Handel TM, Lee PP, Butcher EC, Leung LL
    J. Biol. Chem., 2009;284(2):751-8.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development


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