Human Claudin-17 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4619G-100UG
Human Claudin-17 Alexa Fluor® 488-conjugated Antibody in Data
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Human Claudin-17 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Claudin‑17. Stains human Claudin-17 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 410509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-17
Met1-Val224
Accession # P56750
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
A431 human epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Claudin-17 antibody in HEK293 Human Cell Line Transfected with Human Claudin-17 antibody by Flow Cytometry. View Larger

Detection of Claudin‑17 in HEK293 Human Cell Line Transfected with Human Claudin-17 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Claudin-17 was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human Claudin-17 Monoclonal Antibody (Catalog # IC4619G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-17

Claudin-17 is a 224 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Human Claudin-17 shares 80%‑82% aa sequence identity with mouse and rat Claudin-17.

Entrez Gene IDs
26285 (Human); 239931 (Mouse); 304125 (Rat)
Alternate Names
claudin 17; Claudin17; Claudin-17; CLDN17; human CLDN17 gene for claudin-17, 10claudin-17; MGC126552; MGC126554

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