Human Claudin-17 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4619G-100UG
Detection  of Claudin‑17 in HEK293 Human Cell Line Transfected with Human  Claudin-17 by Flow Cytometry.
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Human Claudin-17 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Claudin‑17. Stains human Claudin-17 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 410509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-17
Met1-Val224
Accession # P56750
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
A431 human epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Claudin‑17 in HEK293 Human Cell Line Transfected with Human  Claudin-17 by Flow Cytometry. View Larger

Detection of Claudin‑17 in HEK293 Human Cell Line Transfected with Human Claudin-17 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Claudin-17 was stained with Alexa Fluor® 488‑conjugated Mouse Anti-Human Claudin‑17 Monoclonal Antibody (Catalog # IC4619G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-17

Claudin-17 is a 224 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Human Claudin-17 shares 80%‑82% aa sequence identity with mouse and rat Claudin-17.

Entrez Gene IDs
26285 (Human); 239931 (Mouse); 304125 (Rat)
Alternate Names
claudin 17; Claudin17; Claudin-17; CLDN17; human CLDN17 gene for claudin-17, 10claudin-17; MGC126552; MGC126554

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