Human Claudin-17 Alexa Fluor® 488-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Claudin‑17. Stains human Claudin-17 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 410509
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Claudin-17
    Met1-Val224
    Accession # P56750
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    A431 human epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Claudin‑17 in HEK293 Human Cell Line Transfected with Human Claudin-17 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Claudin-17 was stained with Alexa Fluor® 488‑conjugated Mouse Anti-Human Claudin‑17 Monoclonal Antibody (Catalog # IC4619G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Claudin-17

Claudin-17 is a 224 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Human Claudin-17 shares 80%‑82% aa sequence identity with mouse and rat Claudin-17.

  • Entrez Gene IDs:
    26285 (Human); 239931 (Mouse); 304125 (Rat)
  • Alternate Names:
    claudin 17; Claudin17; Claudin-17; CLDN17; human CLDN17 gene for claudin-17, 10claudin-17; MGC126552; MGC126554
Related Research Areas

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