Detection of Claudin‑17 in HEK293 Human Cell Line Transfected with Human Claudin-17 by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human Claudin-17 was stained with Mouse Anti-Human Claudin‑17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of Claudin‑17 in A431 Human Cell Line by Flow Cytometry.
A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Claudin‑17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Claudin-17 is a 224 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Human Claudin-17 shares 80%‑82% aa sequence identity with mouse and rat Claudin-17.
Entrez Gene IDs:
26285 (Human); 239931 (Mouse); 304125 (Rat)
claudin 17; Claudin17; Claudin-17; CLDN17; human CLDN17 gene for claudin-17, 10claudin-17; MGC126552; MGC126554
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