Human Claudin‑17 Antibody

Name: Human Claudin‑17 Antibody
Brand: R&D Systems
SKU: MAB4619
Price: 419 USD
Availability: InStock
Rating: 4 (1 reviews)

R&D Systems | Catalog # MAB4619

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Citations (1)

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 410509

View all Claudin-17 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-17
Met1-Val224
Accession # P56750

Specificity

Detects human Claudin‑17. Stains human Claudin-17 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Claudin‑17 Antibody

Detection of Claudin‑17 in HEK293 Human Cell Line Transfected with Human Claudin-17 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human Claudin-17 was stained with Mouse Anti-Human Claudin-17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Detection of Claudin‑17 in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Claudin-17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Claudin‑17 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human Claudin-17

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A431 human epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 1 review rated 4 using MAB4619 in the following applications:

Immunofluorescence (1 Review)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Claudin-17

Claudin-17 is a 224 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Human Claudin-17 shares 80%‑82% aa sequence identity with mouse and rat Claudin-17.