Claudin-3 is a 23 kDa multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-3 is upregulated by EGF and in inflammation and a variety of epithelial cancers. Its expression is lost in the blood brain barrier during pathological disruptions of that structure. Claudin-3 binding to the Clostridium perfringes exotoxin induces epithelial cell lysis. Human Claudin-3 shares 91% amino acid sequence identity with mouse and rat Claudin-3.
Human Claudin‑3 APC‑conjugated Antibody
R&D Systems | Catalog # FAB4620A
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 385021
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Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human Claudin-3
Met1-Val220
Accession # O15551
Met1-Val220
Accession # O15551
Specificity
Stains human Claudin-3 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Claudin‑3 APC‑conjugated Antibody
Detection of Claudin‑3 in PC‑3 Human Cell Line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-3 APC-conjugated Monoclonal Antibody (Catalog # FAB4620A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human Claudin‑3 APC‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: PC‑3 human prostate cancer cell line
Sample: PC‑3 human prostate cancer cell line
Reviewed Applications
Read 1 review rated 5 using FAB4620A in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Claudin-3
Alternate Names
Claudin3, CLDN3, CPE-R 2, CPETR2, HRVP1
Gene Symbol
CLDN3
UniProt
Additional Claudin-3 Products
Product Documents for Human Claudin‑3 APC‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Claudin‑3 APC‑conjugated Antibody
For research use only
Citations for Human Claudin‑3 APC‑conjugated Antibody
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Application: Flow CytometrySample Tested: 293T cellsSpecies: HumanVerified Customer | Posted 12/07/2016HEK293T cell line was transfected with Claudin-3 and stained with Mouse Anti-Human Claudin‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4620A) or APC isotype control.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Blood-Brain Barrier and Immune Cell Transmigration: ICAM-1/CD54 Signaling Pathways
Blood-Brain Barrier and Immune Cell Transmigration: Pathways Overview
Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways
Blood-Brain Barrier and Immune Cell Transmigration: VEGF Signaling Pathways
