Human Claudin-3 Antibody

Catalog # Availability Size / Price Qty
Detection of Claudin‑3 in PC‑3 Human Cell Line by Flow Cytometry.
3 Images
Product Details
Citations (1)
Supplemental Products

Human Claudin-3 Antibody Summary

Species Reactivity
Stains human Claudin-3 transfectants but not irrelevant transfectants.
Monoclonal Mouse IgG2A Clone # 385021
Protein A or G purified from hybridoma culture supernatant
HEK293 human embryonic kidney cell line transfected with human Claudin-3
Accession # O15551
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
2.5 µg/106 cells
See below
8-25 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Claudin‑3 antibody in PC‑3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Claudin‑3 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-3 Monoclonal Antibody (Catalog # MAB4620, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Immunohistochemistry Claudin-3 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

Claudin‑3 in Human Colon. Claudin-3 was detected in immersion fixed paraffin-embedded sections of human colon array using Mouse Anti-Human Claudin-3 Monoclonal Antibody (Catalog # MAB4620) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry Claudin-3 antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC). View Larger

Claudin‑3 in PC‑3 Human Cell Line. Claudin-3 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human Claudin-3 Monoclonal Antibody (Catalog # MAB4620) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-3

Claudin-3 is a 23 kDa multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-3 is upregulated by EGF and in inflammation and a variety of epithelial cancers. Its expression is lost in the blood brain barrier during pathological disruptions of that structure. Claudin-3 binding to the Clostridium perfringes exotoxin induces epithelial cell lysis. Human Claudin-3 shares 91% amino acid sequence identity with mouse and rat Claudin-3.

Entrez Gene IDs
1365 (Human); 12739 (Mouse); 65130 (Rat)
Alternate Names
C7orf1; claudin 3; Claudin3; Claudin-3; CLDN3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-R2; CPETR2; CPETR2CPE-receptor 2; HRVP1; Rat ventral prostate.1 protein homolog; RVP1; ventral prostate.1 protein homolog; ventral prostate.1-like protein

Product Datasheets

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Citation for Human Claudin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor.
    Authors: Im, Hyungsoo, Shao, Huilin, Park, Yong Il, Peterson, Vanessa, Castro, Cesar M, Weissleder, Ralph, Lee, Hakho
    Nat Biotechnol, 2014;32(5):490-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Antibody Profiling


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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