Human CLEC9a Alexa Fluor® 700-conjugated Antibody

Clone 683409 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB6049N
Detection of CLEC9a in HEK293 Human Cell Line Transfected with Human Clec9a and eGFP by Flow Cytometry.
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Citations (1)
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Human CLEC9a Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC9a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 10A, 12B, 14A, rhCD302/CLEC13a, rhMICL, or recombinant mouse CLEC9a is observed.
Source
Monoclonal Mouse IgG1 Clone # 683409
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC9a
Lys57-Val241
Accession # Q6UXN8
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CLEC9a antibody in HEK293 Human Cell Line Transfected with Human Clec9a and eGFP antibody by Flow Cytometry. View Larger

Detection of CLEC9a in HEK293 Human Cell Line Transfected with Human Clec9a and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Clec9a and eGFP was stained with either (A) Mouse Anti-Human CLEC9a Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB6049N) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CLEC9a antibody in Human Peripheral Blood Cells antibody by Flow Cytometry. View Larger

Detection of CLEC9a in Human Peripheral Blood Cells by Flow Cytometry. Human peripheral blood cells gated on CD3-CD141+cells were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (Catalog # FAB4869P) and either (A) Mouse Anti-Human CLEC9a Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB6049N) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CLEC9a

CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C‑type lectin superfamily (1, 2). Mature human CLEC9a consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (3‑5). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (3, 5). Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes (3‑5). BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells (4, 5). CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (3‑5). In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell (7). The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris (7). CLEC9a signaling triggers activation of the tyrosine kinase Syk (3, 7).

References
  1. Huysamen, C. and G.D. Brown (2009) FEMS Microbiol. Lett. 290:121.
  2. Geijtenbeek, T.B.H. et al. (2004) Annu. Rev. Immunol. 22:33.
  3. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
  4. Caminschi, I. et al. (2008) Blood 112:3264.
  5. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
  6. Dudziak, D. et al. (2007) Science 315:107.
  7. Sancho, D. et al. (2009) Nature 458:899.
Long Name
C-type Lectin-like Receptor 9
Entrez Gene IDs
283420 (Human); 232414 (Mouse)
Alternate Names
CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human CLEC9a Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Defining human dendritic cell progenitors by multiparametric flow cytometry.
    Authors: Breton G, Lee J, Liu K, Nussenzweig M
    Nat Protoc, 0;10(9):1407-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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