Human CLEC9a Antibody

Clone 683409 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB6049
MAB6049-SP
Product Details
FAQs
Supplemental Products
Reviews

Human CLEC9a Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC9a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 10A, 12B, 14A, rhCD302/CLEC13a, rhMICL, or recombinant mouse CLEC9a is observed.
Source
Monoclonal Mouse IgG1 Clone # 683409
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC9a
Lys57-Val241
Accession # Q6UXN8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human HEK293 cells transfected with human CLEC9a.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Binding Inhibition

The binding of Recombinant Human CLEC9a Fc Chimera (Catalog # 6049-CL, 100 ng/mL) to a ligand expressed in Fas Ligand-treated A20 mouse B cell lymphoma cell line was maximally inhibited (>90%) by 1 µg/mL of the antibody, as detected by flow cytometry.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC9a

CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C‑type lectin superfamily (1, 2). Mature human CLEC9a consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (3‑5). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (3, 5). Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes (3‑5). BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells (4, 5). CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (3‑5). In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell (7). The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris (7). CLEC9a signaling triggers activation of the tyrosine kinase Syk (3, 7).

References
  1. Huysamen, C. and G.D. Brown (2009) FEMS Microbiol. Lett. 290:121.
  2. Geijtenbeek, T.B.H. et al. (2004) Annu. Rev. Immunol. 22:33.
  3. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
  4. Caminschi, I. et al. (2008) Blood 112:3264.
  5. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
  6. Dudziak, D. et al. (2007) Science 315:107.
  7. Sancho, D. et al. (2009) Nature 458:899.
Long Name
C-type Lectin-like Receptor 9
Entrez Gene IDs
283420 (Human); 232414 (Mouse)
Alternate Names
CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CLEC9a Antibody

There are currently no reviews for this product. Be the first to review Human CLEC9a Antibody and earn rewards!

Have you used Human CLEC9a Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review