Human Coagulation Factor III/Tissue Factor Alexa Fluor® 647-conjugated Antibody Summary
cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
Accession # P13726
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB23391R) or (B) Mouse IgG1 Alexa Fluor 647 Isotype Control (Catalog # IC002R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor, with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).
- Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659.
- Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
- Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
- Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
- Morrissey, J.H. et al. (1987) Cell 50:129.
- Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
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