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Human Coagulation Factor III/Tissue Factor Alexa Fluor® 647-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry

Flow Cytometry

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  • Species Reactivity
    Human
  • Specificity
    Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no
    cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 323519
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III
    Gly34-Glu251
    Accession # P13726
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry Coagulation Factor III/Tissue Factor Antibody (323519) [Alexa Fluor® 647]
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Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB23391R) or (B) Mouse IgG1 Alexa Fluor 647 Isotype Control (Catalog # IC002R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor,  with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).
  • References:
    1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
    2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
    3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
    4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
    5. Morrissey, J.H. et al. (1987) Cell 50:129.
    6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
  • Entrez Gene IDs:
    2152 (Human); 14066 (Mouse)
  • Alternate Names:
    CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

 Ctrl IC002R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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