|Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB23391G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.|
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a 46 kDa integral membrane protein that is part of the extrinsic pathway of coagulation. It functions as a protein cofactor/receptor for Coagulation Factor VII, which is synthesized in the liver and circulates in the plasma (1). Upon binding to TF, the inactive Factor VII is rapidly converted into activated Factor VIIa. The resulting 1:1 complex of Factor VIIa and TF activates the coagulation pathway, and as a soluble form, plays an important coagulation-independent role in processes such as angiognesis (2-4). Synthesized as a 295 amino acid (aa) precursor, TF consists of a signal peptide (aa 1-32) and the mature chain (aa 33-295). The mature region contain an extracellular region (aa 33-251), followed by a transmembrane segment (aa 252-274) and a cytoplasmic tail (aa 275-295) (5-8). TF is found on endothelial cells, monocytes and vascular smooth muscle cells. Over aa 33-251, human and mouse TF share 58% aa sequence identity.
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